Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6790-6793.doi: 10.3969/j.issn.1673-8225.2010.36.031

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Analysis of tumor stem cells-related side population in intestine cancer cell line SW-620 

Gao Gang-long1, Zhang Xue-li1, Liu Wen-yong2, Zhang Ping3, Ye Dong-xia3   

  1. 1 Department of General Surgery, Fengxian Hospital, Southern Medical University (Department of General Surgery, Fengxian Central Hospital), Shanghai  201400, China; 2 Department of General Surgery, 3 Laboratory of Oral Neoplasms, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai  200011, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Zhang Xue-li, Doctor, Chief physician, Department of General Surgery, Fengxian Hospital, Southern Medical University (Department of General Surgery, Fengxian Central Hospital), Shanghai 201400, China lejing1996@yahoo.cn
  • About author:Gao Gang-long★, Master, Physician, Department of General Surgery, Fengxian Hospital, Southern Medical University (Department of General Surgery, Fengxian Central Hospital), Shanghai 201400, China ggl149@sina.com
  • Supported by:

    the Scientific Foundation Program of Health Bureau of Shanghai City, No. 2007157*

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Abstract:

BACKGROUND: In some malignant tumors, side population (SP) cells have the property of tumor stem cells. The studies of human cerebroma and breast carcinoma used this method. At present, studies concerning intestine cancer stem cells are in exploration stage. There is no report on successful isolation and identification of intestine cancer stem cells.
OBJECTIVE: To identify the SP of cancer stem-like cells in intestine cancer cell line SW-620.
METHODS: SW-620 tumor cells suspension was prepared and stained with Hoechst33342 and propidium iodide. In the experimental group, Hoechst33342 was added until the final concentration was 5 mg/L. In the verapamil group, an additional verapamil was added until the final concentration was 5 mg/L. SP was analyzed using flow cytometry. Results from three tests were selected.
RESULTS AND CONCLUSION: Using Hoechst33342 blue and red light double reference graph. In the experimental group, SP cell line located at the lower left quarter region where two kinds of fluorescence were negative or very weak. Following three tests, SP proportion in SW620 was respectively 0.1%, 1.0% and 0.5%. Following verapamil treatment, the SP cell proportion was 0. These indicated that SW620 cell line contains SP cells; i.e. the possibility of SW620 cell line existence. Verapamil can inhibit dye, and obviously decrease the proportion of SP cells.

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