Abstract:

BACKGROUND: From a genetic perspective, the tumor cells and embryonic stem cells (ESCs) are regulated by the proto-oncogene and have amazing ability to divide. Embryonic cancer cells were transplanted into blastocysts of normal development mice and produced chimeric mice without the malignant phenotype. The fate of tumor cells can change with embryonic existence.
OBJECTIVE: To study the effect of mouse ESCs on the changes in biological behaviors of Lewis lung cancer cells.
METHODS: Mouse ESCs were cocultured with Lewis lung cancer cells using Transwell chamber in vitro. In the experimental group, fibroblasts and ESCs were added in the Transwell chamber, whereas tumor cells were in the 12-well plate. In the control group, fibroblasts and fibrocytes were added in the Transwell chamber. The total number of cells were in the chamber did not change, and tumor cells were in the 12-well plate.
RESULTS AND CONCLUSION: Compared with the control group, the experimental group shows significant difference in morphology of Lewis lung cancer cells; some cells appear signs of aging and apoptosis. The proliferation of Lewis lung cancer cells was significantly decreased (P < 0.05), and the number of Lewis lung cancer cells that could traverse DB gel biomembrane was significantly reduced in the experimental group (P < 0.05). Results indicate that ESCs can inhibit the growth, proliferation and invasiveness of Lewis lung cancer cells.