Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (20): 3714-3717.doi: 10.3969/j.issn.1673-8225.2010.20.024

Previous Articles     Next Articles

Screening of interactional partners of human heterogeneous ribonucleo protein E1 using yeast two-hybrid system 3

Huo Li-rong1,2, Zou Jun-hua3, Zhong Nan3   

  1. 1Department of Physiology, School of Basic Medical Sciences, Capital Medical University, Beijing  100069, China;
    2Department of Microbiology & Immunology, Preclinical Medical College of Shanxi Medical University, Taiyuan  030001, Shanxi Province, China;
    3Department of Heredity, School of Basic Medical Sciences, Peking University, Beijing  100083, China
  • Online:2010-05-14 Published:2010-05-14
  • About author:Huo Li-rong, Doctor, Associate professor, Department of Physiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China; Department of Microbiology & Immunology, Preclinical Medical College of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China huolr002@126.com
  • Supported by:

    Youth Foundation of Shanxi Medical University, No. 02200805*

PDF

334

Abstract:

BACKGROUND: Now yeast two-hybrid system 3 is a common platform applied in screening interactive proteins.

OBJECTIVE: To screen interactional partners with human heterogeneous ribonucleo protein E1 (hnRNP E1) using yeast two-hybrid system 3.

METHODS: The carrier of hnRNP E1-pGBKT7/c-myc were constructed. Then AH109 yeast cells were transformed with it, and the expression of hnRNP E1 in yeast cells was identified by Western blot. After toxicity and self-activation assays, yeast-mating was utilized to screen interactional proteins with hnRNP E1 in human cDNA library including nutritional deletion, filtration membrane reaction and yeast mating. 

RESULTS AND CONCLUSION: hnRNP E1 protein was expressed normally and there was no self-activation and toxicity in AH109. Sixteen candidate genes were screened after 3 passages and filter assay. And eight candidate proteins were gained at last after BLASTN/P from NCBI. All results revealed that hnRNP E1 maybe participate in the process of replication, transcription, transport for mRNA, cell cycle, cell autophagy and immune response, and it can participate in the pathogenesis of some genetic disorders.

CLC Number: