Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (20): 3705-3708.doi: 10.3969/j.issn.1673-8225.2010.20.022

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Preparation of reflux esophagitis rat models: Partial pylorus ligation plus forestomach ligation with different diameters of stents

Zheng Chao-xu1, Lin Wei-bin2, Yu Jun-feng1, Chen Liu-hua1   

  1. 1Department of Minimally Invasive Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China;   
    2Department of General Surgery, Hexian Memorial Hospital, Panyu District, Guangzhou 511400, Guangdong Province, China
  • Online:2010-05-14 Published:2010-05-14
  • About author:Zheng Chao-xu, Doctor, Associate chief physician, Master’s supervisor, Department of Minimally Invasive Surgery, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China zhengchaoxu@yahoo.com
  • Supported by:

    the Science and Technology Planning Project of Guangdong Province, No. 2005 B31201015*

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Abstract:

BACKGROUND: Partial pylorus ligation is a common method for establishment of reflux esophagitis (RE) model. The diameter of pylorus ligation is closely related to the efficacy of establishment.

OBJECTIVE: To investigate the diversity of partial pylorus ligation with different diameters of stents plus forestomach ligation and to find a rational method to induce RE in rats.

MATERIALS: Forty female Wistar rats were randomly divided into 2 groups, and 2.9 mm diameter and 3.7 mm diameter stents were inserted through the incision to sustain the pylorus and extracted after pylorus ligation. Then ligation of the transitional region between the forestomach and stomach was performed additionally to establish RE model. The body weights and 7-day survival rates of rats in each group were observed. The rats were sacrificed 7 days after the procedures, and the incidences of esophagitis of two groups were counted.

RESULTS AND CONCLUSION: The 7-day survival rates of 2.9 mm diameter and 3.7 mm diameter stents groups were 35% (7/20) and 68.4% (13/19), respectively (P = 0.038). The incidences of esophagitis were 85.7% (6/7) and 76.9% (10/13), respectively. There were no significant differences in the body weights of everyday postoperatively in survival rats between two groups (P > 0.05). Successful RE model in rats can be established by partial pylorus ligation plus forestomach ligation. The pylorus ligation diameter is closely related to the survival rates of rats.

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