Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (20): 3696-3700.doi: 10.3969/j.issn.1673-8225.2010.20.020

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Establishment of human colonic carcinoma (LoVo) xenograft in nude mice and magnetic resonance imaging examination  

Feng Shi-ting1, Sun Can-hui1, Cai Hua-song1, Zhou Jian1, Tan Guo-sheng1, Qiu Peng-xin2, Zhang Jian3, Li Zi-ping1, Meng Quan-fei1   

  1. 1Department of Radiology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China;
    2Department of Pharmacology, 3Laboratory Animal Center, Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Online:2010-05-14 Published:2010-05-14
  • Contact: Li Zi-ping, Doctor, Professor, Department of Radiology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China Liziping@tom.com
  • About author:Feng Shi-ting, Studying for doctorate, Attending physician, Department of Radiology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Supported by:

    Technology Planning Project of Guangdong Province, No. 2007B031516007*

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Abstract:

BACKGROUND: The colonic carcinoma xenograft model is an ideal method for exploring and stimulating in vivo biological behavior of human, however, studies concerning evaluating models using magnetic resonance imaging (MRI) have less been reported yet.

OBJECTIVE: To establish human colonic carcinoma xenograft model in nude mice with human colonic carcinoma LoVo cells inoculation and transplantation, and to evaluate the models by using MRI. 

METHODS: The nude mice models with colonic carcinoma were established by cell transplantation and tumor transplantation. The morphological and histopathological features of transplanted tumor were examined. Blood samples taken from the host were assayed for carcino embryonic antigen levels. Immunohistochemical localization of carcino embryonic antigen in the tumor tissues was also determined. The models (n = 6) were performed MRI examination. Signal to noise ratio (SNR) on T1 weighted imaging (T1WI) and T2 weighted imaging (T2WI), T1 and T2 relaxation time of the tumor, liver and muscle were measured.

RESULTS AND CONCLUSION: It showed that the morphological and functional characteristics of the transplanted tumors were strikingly identical to the original human tumor. The successful transplantation rate was 100%. The mean of SNR on T1WI of tumor, liver and muscle were 26.19, 22.71, and 26.62, respectively, and, the mean of SNR on T2WI were 9.42, 7.66 and 8.59, respectively. T1 relaxation time of tumor, liver and muscle were 1 039.22 ms, 907.63 ms and 1 611.51 ms, respectively, while the mean of T2 relaxation time were 109.95 ms, 37.31 ms and 64.35 ms, respectively. MRI was performed successfully in all the models with good imaging quality and high resolution. T1 and T2 relaxation time could be the quantitative index for further evaluation.

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