Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (20): 3678-3681.doi: 10.3969/j.issn.1673-8225.2010.20.016

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Effect of Zhuangjin Xugu decoction on tibial fracture healing: A verification of core binding factor alpha-1 expression using reverse transcription-polymerase chain reaction

Wang Li1, Zheng Su1, Yang Feng-yun1, Wang Li-hua1, Bao Jie-wei2   

  1. 1Department of Orthopedic Joints, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang   330006, Jiangxi Province, China;
    2Jiangxi University of Traditional Chinese Medicine, Nanchang   330006, Jiangxi Province, China
  • Online:2010-05-14 Published:2010-05-14
  • About author:Wang Li, Doctor, Associate chief physician, Department of Orthopedic Joints, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, Jiangxi Province, China wangli0791@sina.com
  • Supported by:

    the Science and Technology Project of Jiangxi Province Department of Education in 2007, No. [2007]254*

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Abstract:

BACKGROUND: Previous research proved that Chinese herbs could promote bone fracture healing at levels of pathological section, biomechanics, and biochemical test.

OBJECTIVE: To probe into the effect of Zhuangjin Xugu decoction recipe on the expression of core binding factor α1 (Cbfα1) during tibial fracture healing.

METHODS: The model of closed fracture fixation was induced in marrow of 100 SD male rats aging 3 months, and the rats were then randomly divided into Zhuangjin Xugu decoction recipe group and control group. Rats in the Zhuangjin Xugu decoctiongroup were continued to take Zhuangjin Xugu decoction, 2 mL per administration, twice a day. Rats in the control group were given the same volume of normal saline. Semi-quantitative analysis was used to detect the expression of Cbfα1 of bone tissues in regional fracture in the 1st, 2nd, 4th, 8th, and 12th weeks using RT-PCR method.

RESULTS AND CONCLUSION: The Cbfα1 mRNA was detected at 2 weeks after bone fracture and gradually increased. The expression in the Zhuangjin Xugu decoctiongroup was significantly higher than in the control group (P < 0.05). Zhuangjin Xugu decoction could increase the expression of Cbfα1 during tibial fracture healing.

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