Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (20): 3669-3673.doi: 10.3969/j.issn.1673-8225.2010.20.014

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Feasibility of reducing myocardial infarct area using intravenous injection of platelet-derived growth factor plasmid

Wang Jian1,2,3, Yao Yao1,2,3, Li Dan1,2,3, Shen Xiao-tao1,2,3,4, Zheng Xin1,2,3, Liu Shao-kun1,2,3, Liu Juan-juan1,2,3, Cai Dong-qing1,2,3,4   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3the International Science & Technology Cooperation Base, Guangdong Provincial Department of Science and Technology, 4Department of Biomedical Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Online:2010-05-14 Published:2010-05-14
  • Contact: Cai Dong-qing, Doctor, Professor, Doctoral supervisor, Key Laboratory for Regenerative Medicine, Ministry of Education, Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, the International Science & Technology Cooperation Base, Guangdong Provincial Department of Science and Technology, Department of Biomedical Engineering, Jinan University, Guangzhou 510632, Guangdong Province, China tdongbme@jnu.edu.cn
  • About author:Wang Jian, Studying for master’s degree, Key Laboratory for Regenerative Medicine, Ministry of Education, Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, the International Science & Technology Cooperation Base, Guangdong Provincial Department of Science and Technology, Jinan University, Guangzhou 510632, Guangdong Province, China wangjian6796298@163.com
  • Supported by:

    the 863 Program, No. 2007AA02Z105*;
    the National Natural Science Foundation of China, No. 30770886*, 30570369*, 30340038*, 30973158*;
    Guangdong Key Grant for Natural Science Foundation, No. 04105826*;
    Guangdong Grant for Science and Technology Development, No. 2004B30601007*;
    Foundation for the Chinese Scholars Returned from Oversea, Ministry of Education and Guangzhou Grant for Science and Technology Development, No. 2006Z3-E5251*

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Abstract:

BACKGROUND: Previous studies have demonstrated that intramyocardial injection of platelet-derived growth factor (PDGF) and its encoded gene vector prior to or immediately after myocardial ischemia is able to decrease myocardial infarcted areas. However, whether intravenous injection of PDGF gene vector after myocardial infarction has the same effect is still unclear.

OBJECTIVE: To investigate the effect of intravenous injection of phPDGF-B plasmid on infarct areas after myocardial infarction.

METHODS: Twenty Sprague Dawley rats were prepared for myocardial infarction models and randomly divided into 4 groups. The phPDGF-B plasmid was intravenously injected into the male and female experimental groups at 30 minutes after ligation. In the male, female control groups, the same volume of phosphate buffered saline was injected. The Masson Trichrome staining was performed at 2 weeks after operation, and the infarcted area, the infarcted zone fibrosis and the geometry of left ventricle were analyzed.

RESULTS AND CONCLUSION: The infarcted areas of the experimental groups were less than that of the control groups at 2 weeks after injection (P < 0.05). Compared with the control groups, a lot of viable cells were found in infarcted zone in the experimental groups. Even though, the area of the fibrosis in the experimental groups was less than control groups, however, the difference had no statistical significance. In addition, the collagen deposition density in the experimental groups was less than control groups. Furthermore, it was found that the wall thickness of border zone in left ventricle of experimental groups was longer than control groups (P < 0.05); while the thickness of infarcted myocardium in left ventricle was greater than that of the control group. All results demonstrated that intravenous delivery of phPDGF-B plasmid had a protective effect on the myocytes of infarcted area, which can decrease the infarction size of ischemic myocardium, improve the reconstruction of left ventricle geometry and reduce the fibrosis of infarcted area after myocardial infarction. This effect has no difference on different genders

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