Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (20): 3629-3632.doi: 10.3969/j.issn.1673-8225.2010.20.005

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Effects of sequential application of estrogen and simvastatin therapy on osteoporosis in ovariectomized rats

Ma Qing-fen1, Yao Zhen-wei2, Shan Ti-xin1   

  1. 1Department of Gynecology, Peoples’ Hospital of Shouguang City, Shouguang  262700, Shandong Province, China;
    2First Affiliated Hospital of Chongqing Medical University, Chongqing  400016, China
  • Online:2010-05-14 Published:2010-05-14
  • About author:Ma Qing-fen, Master, Physician, Department of Gynecology, Peoples’ Hospital of Shouguang City, Shouguang 262700, Shandong Province, China mqf0408@163.com

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Abstract:

BACKGROUND: Frost founded the theory of bone remodeling intervention, named sequential application, which administrate a promoting bone drug followed by an inhibitor of bone resorption.

OBJECTIVE: According to the bone remodeling intervention theory, to investigate the effects of sequential application of estrogne and simvastatin on osteoporosis in ovariectomized rats.

METHODS: Totally 40 SD male rats with 3-month-old were randomly divided into ovariectomized group and normal control group. In the ovariectomized group, double ovaries of rats were removed. In the normal control group, only the hypogastrium skins of rats were exposed. At 1 month after operation, the ovariectomized group were randomly allocated into sequential estradiol benzoate and simvastatin (sequential group), estrogen group and ovariectomized control group, and received medicine intervention as follows: coherent group: subcutaneous injected estradiol benzoate (0.1 mg/kg) per triduum for 2 weeks, followed by intragastric administrated simvastatin (5 mg/kg per day) for 2 weeks, the medication was removed for 5 weeks, and then intragastric administrated simvastatin (5 mg/kg per day) for 2 weeks; estrogen group: subcutaneous injected estradiol benzoate (0.1 mg/kg) per triduum for 11 weeks; in the ovariectomized control group, rats were given animal feeds only. After 11 weeks, the bone mineral density (BMD) was measured by double X-ray densitometry, and interleukin-6 (IL-6) and osteocalcin levels were detected by radioimmunoassay.

RESULTS AND CONCLUSION: The BMD and osteocalcin levels were greater in treated groups than the ovariectomized control group (P < 0.05); especially higher in the sequential group than the estrogen group (P < 0.05). Meantime, the expression of IL-6 in the treated groups were degraded compared to the ovariectomized control group (P < 0.05), which was lower in the sequential group than the estrogen group (P < 0.05). The results demonstrated that sequential application of estrogen and simvastatin can prevent osteoporosis by inhibiting bone resorption and improving bone formation.

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