Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (20): 3620-3624.doi: 10.3969/j.issn.1673-8225.2010.20.003

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Influence of nivalenol on aggrecan synthesis of cultured chondrocytes

Cao Pei-hua1, Cao Jun-ling2, Chen Jing-hong2, Yang Ya-juan1   

  1. 1Department of Clinical Laboratory Medicine, Xi’an Medical University, Xi’an  710021, Shaanxi Province, China;
    2Institute of Endemic Diseases, Environment Related Gene Key Laboratory of Ministry of Education, Xi’an Jiaotong University, Xi’an  710061, Shaanxi Province, China
  • Online:2010-05-14 Published:2010-05-14
  • Contact: Cao Jun-ling, Professor, Doctoral supervisor, Institute of Endemic Diseases, Environment Related Gene Key Laboratory of Ministry of Education, Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China caojl@mail.xjtu.edu.cn
  • About author:曹珮华，女，1974年生，陕西省西安市人，汉族，2006年西安交通大学医学院毕业，硕士，讲师，主要从事生物化学与分子生物学检验的教学工作。 caoph@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872187*;
    Foundation of Xi’an Medical University, No. 2007FZ04*

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Abstract:

BACKGROUND: Nivalenol (NIV) toxin is one of the mycotoxins, which is supposed to probably contribute to the classical features of Kashin-beck disease.
OBJECTIVE: To investigate the influence of NIV on the expression of aggrecan in the cultured chondrocytes.
METHODS: Human chondrocytes cultured in vitro were treated with NIV at different concentrations for varied time periods (1-    5 days), and the cell viability was measured by MTT assay. DNA content was determined by UV Spectrophotometry. Aggrecan mRNA expression was studied by semiquantitative RT-PCR. The protein expression of aggrecan was examined by using Western blot analysis.
RESULTS AND CONCLUSION: NIV at a dose of 0.025 mg/L could stimulate chondrocytes growth in a tendency of increasing followed by decreasing. The chondrocytes viability was initially increased when treated with NIV, which would be suppressed by toxin when treated with NIV at a dose of 0.05-0.1 mg/L. When treated with more than 0.2 mg/L NIV, the chondrocytes viability was obviously decreased with concentration increasing and time prolonging. The NIV with concentration of 0.1-0.5 mg/L inhibited aggrecan synthesis and reduced its mRNA expression. The results demonstrated that NIV significantly decrease aggrecan synthesis in the chondrocytes and reduced its mRNA expression. NIV could inhibit chondrocyte proliferation in a dose- and time-dependent manner. 

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