Abstract:

BACKGROUND: The essence of cell differentiation is a selectively intra-cellular gene expression, which results in specific proteinic synthesis and causes changes in biochemistry, structure and function. Thus, original proteomics and a single protein analysis can not meet the requirement in study. Proteomics technology provides a powerful tool due to the large scale, systemical study of protein transformation and interaction, which can be used for exploring molecular mechanism of bone mesenchymal stem cells (BMSCs) during directional differentiation.
OBJECTIVE: To introduce proteomics, to summarize the research of proteomics in directional differentiation of BMSCs, and to forecast the development of proteomics research methods.
METHODS: To search articles highly related with BMSCs, cell differentiation, and proteomics published on CNKI (www.cnki.net/index.htm), Sciencedirect (http://www.Sciencedirect.com), I.S.I (http://www.isiwebofknowledge.com) were searched, and the key achievements were included in the analysis.
RESULTS AND CONCLUSION: A total of 29 documents were reviewed, and the experiences in the application of proteomics technology in the directional differentiation of BMSCs were summarized. With the innovation and development in methodology and technology, proteomics will become a powerful tool for us to study the potential mechanisms of BMSCs directional differentiation.