Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (14): 2523-2527.doi: 10.3969/j.issn.1673-8225.2010.14.011

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Effect of hyperbaric oxygen on differentiation and Wnt3 expression of bone marrow mesenchymal stem cells

Chen Chong-feng, Yang Yu-jia, Yao Yue, Wang Qing-hong, Li Meng   

  1. Department of Pediatrics, Xiangya Hospital, Central South University, Changsha   410008, Hunan Province, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Yang Yu-jia, Doctor, Professor, Doctoral supervisor, Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China yyjcjcp@163.com
  • About author:Chen Chong-feng☆, Studying for doctorate, Department of Pediatrics, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China chenchfeng@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30672240*

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Abstract:

BACKGROUND: Hyperbaric oxygen (HBO) treatment promotes the proliferation and differentiation of endogenous neural stem cells in neonatal rats following hypoxic/ischemic brain damage (HIBD). The Wnt signaling pathway is associated with neurogenesis. However, there are few data recording the role of HBO in the differentiation of neural stem cells in vitro.
OBJECTIVE: To observe the effect of HBO on differentiation and Wnt3 expression of bone marrow mesenchymal stem cells (BMSCs).
METHODS: BMSCs were isolated and cultured. The rat BMSCs of passages 3-5 were cultured in DMEM/F12 (1: 1) medium with basic fibroblast growth factor, epidermal growth factor and B27 for 24 hours. The induced BMSCs were randomly divided into two groups: control group (no treatment) and HBO group (HBO, 0.10 MPa, 60 minutes stabilizing pressure with at least 90% oxygen). The neuron specific encloase (NSE), glial fibrillary acidic protein (GFAP) and O4 marked oligodendrocyte immunocytochemistry were detected by immunofluorescent staining, and Wnt3 protein expression was detected by Western-blot.   
RESULTS AND CONCLUSION: BMSCs cultured in classic medium of neural stem cells could significantly induce the expression of nestin. The expression of NSE and O4 of HBO group was greater than control group (P < 0.01), but GFAP expression displayed no significant difference between the groups (P > 0.05). Western blot showed HBO could enhance the Wnt3 expression (P < 0.05). Results show that HBO can induce BMSCs to differentiate into neural cells and oligodendrocyte, which is correlated with the activation of the Wnt3 protein.

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