Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (14): 2518-2522.doi: 10.3969/j.issn.1673-8225.2010.14.010　

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Expression of pDsRed-C1-CDNF eukaryotic expression vector in rat bone marrow mesenchymal stem cells

Zhang Jun 1, 2, Niu Chao-shi1, Gao Ge1, Tang Shen-feng2, Li Jing2   

  1. 1 Department of Neurosrugery, Anhui Provincial Hospital, Anhui Medical University, Hefei   230001, Anhui Province, China; 2 Molecular Neurobiology & Neural Regeneration and Repairing Institute of Anhui Provincial Hospital, Anhui Medical University, Hefei   230001, Anhui Province, China
  • Online:2010-04-02 Published:2010-04-02
  • About author:Zhang Jun★, Studying for master’s degree, Physician, Department of Neurosrugery, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China; Molecular Neurobiology & Neural Regeneration and Repairing Institute of Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China zhangjun.0210 @163.com.
  • Supported by:

    the Talent Development Foundation Program of Anhui Province, No. 2006Z037*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (MSCs) are a kind of adult stem cells with multi-potential differentiation property. At present, it has served as cell carrier for the treatment of Parkinson's disease.
OBJECTIVE: To construct pDsRed-C1-CDNF eukaryotic expression vector and induce its expression in rat MSCs.
METHODS: CDNF gene was amplified from mouse tissues using RT-PCR, and sequence with Xho I, BamHI restriction enzyme cutting site. The CDNF gene was inserted into the eukaryotic expression vector pDsRed-C1 encoding red fluorescent protein gene. The plasmid pDsRed-C1-CDNF was constructed and transfected into rat bone marrow MSCs.
RESULES AND CONCLUSION: The pDsRed -C1-CDNF recombinant plasmid was confirmed by double digestion of Xho I and BamHI restriction enzyme or single digestion of BamHI, and PCR sequence. Results show that the recombinant pDsRed-C1-CDNF eukaryotic expression vector was successfully constructed.

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