Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (14): 2513-2517.doi: 10.3969/j.issn.1673-8225.2010.14.009

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Superparamagnetic iron oxide and chlormethylbenzamido-1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine labeling and tracing bone marrow mesenchymal stem cells 

Dou Xing-kui1, Guo Tao2, Si Yong-yu1, Wan Lin-jun1, Huang Qing-qing1, Kang Bo2   

  1. 1 Department of Anesthesiology, Second Affiliated Hospital of Kunming Medical College, Kunming  650101, Yunnan Province, China; 2 Department of Cardiology, First Affiliated Hospital of Kunming Medical College, Kunming  650000, Yunnan Province, China
  • Online:2010-04-02 Published:2010-04-02
  • Contact: Guo Tao, Master, Professor, Department of Cardiology, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China guotao20@hotmail.com
  • About author:Dou Xing-kui☆, Studying for doctorate, Attending physician, Department of Anesthesiology, Second Affiliated Hospital of Kunming Medical College, Kunming 650101, Yunnan Province, China douxk@126.com
  • Supported by:

    Specific Research Project of Yunnan Science and Technology Bureau, No. 2008CD007*

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Abstract:

BACKGROUND: The success of cell therapy will depend on the ability to monitor the fate of transplanted cells in vivo. Superparamagnetic iron oxide (SPIO) labeling is an ideal magnetic resonance contrast medium, and it offers the potential for non-invasive tracking of implanted cells. The chlormethylbenzamido-1,1' - dioctadecyl- 3, 3, 3',3' - tetramethylindocarbocyanine (CM-DiI) labeling does not have cytotoxicity, so it cannot influence cell growth.
OBJECTIVE: To investigate the effects of SPIO and CM-DiI labeling and tracking on bone marrow mesenchymal stem cells (BMSCs).
METHODS: Porcine BMSCs were isolated and cultured by the whole bone marrow method. BMSCs were labeled with SPIO containing 50 mg/L Ferrum and CM-DiI. The labeled BMSCs were transplanted into porcine myocardial infarction model via intracoronary infusion. The frozen sections of the cardiac tissues were obtained after 4 weeks.
RESULTS AND CONCLUSION: Efficiency of SPIO and CM-DiI labeling BMSCs was nearly 100%. The SPIO and CM-DiI labeled BMSCs could be found in the cardiac muscle tissues at 4 weeks after transplantation. SPIO and CM-DiI labeling BMSCs were efficiently tracked in vivo.

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