Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (11): 1959-1962.doi: 10.3969/j.issn.1673-8225.2010.11.015

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Establishment and evaluation of an animal model of stress urinary incontinence

Wang Hao-yu1, Du Xiao-wen2, Xu Jian-wei1, Zhu Yong-feng3, Wu Hui-ling3, Hu Jun-biao3, Jin Fan3, Lü Rui-ping3   

  1. 1Department of Plastic Surgery, Zhongshan Hospital of Fudan University, Shanghai   200032, China;
    2Department of Urinary Surgery, Jinhua People’s Hospital, Jinhua   321000, Zhejiang Province, China;
    3Department of Urinary Surgery, Zhongshan Hospital of Fudan University, Shanghai   200032, China
  • Online:2010-03-12 Published:2010-03-12
  • Contact: Xu Jian-wei, Chief physician, Department of Plastic Surgery, Zhongshan Hospital of Fudan University, Shanghai 200032, China xu.jianwei@zs-hospital.sh.cn

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Abstract:

BACKGROUND: Although drug treatment, physics-behavior therapy, and postoperative therapy have been commonly used to treat stress urinary incontinence (SUI), there is still no satisfactory treatment at present.
OBJECTIVE: To build a stable animal model simulating stress urinary incontinence (SUI) by bilateral transaction of pudendal nerve and nerves innervating pelvic floor muscles, including iliococcygeous muscle and pubococcygeous muscle.
METHODS: A total of 18 6-week-old female SD rats weighing (199.44±8.41) g were randomly divided into 3 groups: normal, model, and sham-surgery groups, with 6 rats in each group. Rats in the model group underwent bilateral transaction of pudendal nerves and nerves innervating iliococcygeous/pubococcygeous muscles, while rats in the sham-surgery group had same procedures except nerve transaction. The normal group did not undergo any operation. Each rat was subjected to measure leak point pressure (LPP) at 2 weeks after the operation. After the measurement of LPP, cross sections of connection area of bladder and urethra were sent to histology.
RESULTS AND CONCLUSION: One rat in the sham-surgery group died at 1 week after the operation. The LPP of model group decreased significantly by approximately 33% compared with the normal group (P < 0.05); however, there was no significant difference in LPP between sham-surgery and normal groups (P > 0.05). The results of histology showed loosely arrangement and atrophy of urethral striated muscle fibers in rats of the model group. Bilateral transaction of pudendal nerves and nerves innervating to iliococcygeous/ pubococcygeous muscles resulted in SUI in rats stably.

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