Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (5): 848-853.doi: 10.3969/j.issn.1673-8225.2010.05.021

Previous Articles     Next Articles

Construction of mouse interleukin-10 recombinant adenovirus vector and gene modification for dendritic cells

Xu Fei, Chen Chuan-hui, Lin Yao-guang, Zhang Wei   

  1. Department of Respiratory Medicine, First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-01-29 Published:2010-01-29
  • Contact: Zhang Wei, Professor, Chief physician, Department of Respiratory Medicine, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China zhangweiliuxin@163.com
  • About author:Xu Fei☆, Doctor, Associate professor, Associate chief physician, Department of Respiratory Medicine, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China hiphopchen1984@ sina.com
  • Supported by:

    National Key Technology R&D Program, No. 2008BAI68B00*

PDF

516

Abstract:

BACKGROUND: Few reports concern the effects of dendritic cells-a kind of antigen presenting cells, and interleukin-10 (IL-10) on airway hyperreactivity or inflammation.
OBJECTIVE: To construct mice IL-10 recombinant adenovirus vector Ad-mIL-10 to acquire the dendritic cells modified by mIL-10, which can provide a foundation for the further study.
METHODS: Mouse IL-10 (mIL-10) gene comprise of enzyme cutting spot was synthesized according to the mIL-10 gene sequence and multiclone spot of adenovirus vector, connected to pMD18-T vector and sequenced. MIL-10 was subcloned to BD Adeno-XTM vector, packed and augmented in HEK 293 cells, following determine the protein expression, and the vector was transfected to mice bone marrow-derived dendritic cells.
RESULTS AND CONCLUSION: Recombinant adenovirus vector Ad-mIL-10 was successfully synthesized, packed and augmented, which could highly express protein IL-10. Bone marrow-derived dendritic cells were successfully cultured and transduced in vitro. It suggested that it is feasible to transfect mice dendritic cells by Ad-mIL-10 adenovirus vector. The study can provide more sufficient theoretic evidence for the possibility of correlative gene therapy.

CLC Number: