Effects of repeated superovulation on developmental potential of oocytes in mice and humans

doi:10.12307/2024.125

Abstract

Abstract: BACKGROUND: Superovulation is a common therapy in assisted reproductive technology. In clinical practice, some patients experience repeated superovulation to get pregnant.
OBJECTIVE: To explore the effect of repeated superovulation on the developmental potential of oocytes in mice and humans.
METHODS: Both animal experiments and retrospective clinical research were conducted. The animal study involved 90 SPF grade ICR 8-week-old female mice, who were randomly divided into three groups for 1, 3, and 5 superovulations, respectively. The clinical study involved 306 patients who had undergone three consecutive in vitro fertilization cycles. The number of ovules obtained and embryonic development in different cycles were compared.
RESULTS AND CONCLUSION: (1) The animal study indicated that repeated superovulation did not affect the embryonic development or developmental speed of mouse embryos. Similarly, there was no significant difference in the mouse blastocyst apoptosis, DNA damage, or the formation of inner cell mass and trophectoderm (P > 0.05). (2) The clinical study also revealed no significant differences in the number of retrieved oocytes (8.60±5.04, 8.58±4.87, and 8.38±4.63, P=0.81) and transferable embryos (2.42±1.99, 2.40±1.92, and 2.64±2.00, P=0.26) over the three cycles. (3) In both the young group (< 35 years) and the old group (≥ 35 years), the embryo quality was not affected by repeated superovulation (P > 0.05). (4) These findings show that repeated superovulation does not affect the developmental potential of oocytes in mice and humans.

Key words: repeated superovulation, in vitro fertilization, oocyte, developmental potential

CLC Number:

Li Chong, Shen Xiaoli, Yang Jingwei, Guo Jing, Xie Juan, Huang Guoning, Li Jingyu. Effects of repeated superovulation on developmental potential of oocytes in mice and humans[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(19): 3018-3023.

Figures/Tables 6

References

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