Strategic thinking of human oocyte cryopreservation

doi:10.3969/j.issn.2095-4344.2015.19.021

Abstract

Abstract:

BACKGROUND: Because of the special and complicated biological characteristics of oocytes, the suitable cyropreservation technology for oocytes faces more various challenges. However, the uneven survival rate and fertilization, damages of developmental potential of the thawed oocyte still exist.
OBJECTIVE: To introduce the progress in basic and application researches of oocyte cryopreservation technology, and to illuminate the technical defects and thoughts and possible research points to overcome these problems.
METHODS: A computer-based search of CNKI and PubMed was performed for articles concerning oocyte cryopreservation from January 2004 to October 2014. The search terms were “oocyte, cryopreservation, vitrification” in Chinese and English, respectively. The old and repeated articles were excluded. Finally, 41 articles were included in result analysis.
RESULTS AND CONCLUSION: The cryopreservation of oocytes by slow freezing and vitrification has been used in clinic. At the same time, the uneven survival and fertilization rate, damaged developmental potential of the thawed oocyte still puzzle the clinicians. The key point to breakthrough or improvement of oocyte cryopreservation technology is the systematization of the protocol for oocylte cryopreservation, for example, the choice of cryoprotective agents, the development of carriers for oocytes, and the determination of oocyte stage. Furthermore, other related technologies should also be considered, including the in vitro mature technology of oocytes, cryopreservation and transplantation of ovary tissues.

Key words: Ovum, Oocytes, Cryopreservation

CLC Number:

R318

Wang Pei-tao, Shao Cui-hua, Liu Hai-ning. Strategic thinking of human oocyte cryopreservation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 3074-3082.

Figures/Tables 1

References

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