Changes in periapical tissue of type 2 diabetic mice during tooth occlusal elongation

doi:10.12307/2023.820

Abstract

Abstract: BACKGROUND: In clinical work, some diabetic patients often have tooth loss. If the tooth loss is not timely repaired, it will lead to the elongation of the maxillary teeth, resulting in the reduction of the vertical occlusal gap, early occlusal contact, occlusal interference, and even tooth extraction.
OBJECTIVE: To observe the expression of receptor for advanced glycation end products (RAGE) and bone sialoprotein (BSP) in periapical tissue of diabetic mice during tooth occlusal elongation, and explore the effect of diabetes on periapical tissues.
METHODS: Totally 120 male C57BL mice were divided randomly into diabetic group (n=40), negative control group (n=40) and blank control group (n=40). Animal models of type II diabetes were established in the diabetic group by intraperitoneal injection of 70 mg/kg streptozotocin. The negative control group was intraperitoneally injected with the same amount of sodium citrate solution, while the blank control group was not disposed. After modeling, 30 mice randomly were selected from each group to extract the right maxillary molars. Then, the mice were further divided into five subgroups of 0, 3, 6, 9 and 12 days (n=6 per group). The mice were killed respectively according to the time specified by each group to obtain their right mandibles. The mRNA and protein expression levels of RAGE and BSP in periapical tissue were detected by real-time PCR and immunohistochemistry, respectively.
RESULTS AND CONCLUSION: Real-time PCR results indicated significantly higher RAGE and lower BSP relative mRNA expression levels in the diabetic group than the blank and negative control groups (P < 0.05), while there was no significant difference between blank and negative control groups (P > 0.05). With the time extension of tooth extraction, the relative expression levels of two genes in the three groups both showed a trend of continuous growth. In the diabetic group, the expression levels increased obviously from 3 to 6 days, while in the blank and negative control groups, the expression levels raised apparently from 6 to 9 days. The increase trend of RAGE mRNA relative expression in the diabetic group was more obvious than that in the blank and negative control groups, while the increase trend of BSP mRNA relative expression did the opposite. Immunohistochemical staining results showed that the positive rates of RAGE and BSP in the periapical tissue of the three groups increased with the time prolongation of tooth extraction. The positive rate of RAGE was significantly higher in the diabetic group than the blank and negative control groups at each time after tooth extraction (P < 0.05). The positive rate of BSP was significantly lower in the diabetic group than the blank and negative control groups at 6, 9 and 12 days after tooth extraction (P < 0.05). There was no significant difference in the positive rate of RAGE and BSP between the blank and negative control groups (P > 0.05). To conclude, the expressions of RAGE and BSP in the periapical tissues of diabetic mice increased gradually with the time extension of tooth extraction, while he growth rate and expression of BSP were lower in the diabetic group than the negative and blank control groups. These indicate that diabetes may inhibit the expression of BSP. However, the high expression level of RAGE may be related to the accelerated damage to periapical tissue and delayed tissue reconstruction in diabetes.

Key words: bone sialoprotein, diabetic mouse, receptor for advanced glycation end products, tooth elongation, immunohistochemical staining, real-time fluorescence quantitative PCR

CLC Number:

Zheng Yi, Wei Zhoudan, Guo Dong, Shi Ziqing, Niu Weiran, Xu Zhuoyu, Li Pengcui, Li Wenjin. Changes in periapical tissue of type 2 diabetic mice during tooth occlusal elongation[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(32): 5196-5202.

Figures/Tables 14

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