Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 5971-5975.doi: 10.3969/j.issn.1673-8225.2010.32.018

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Safety of intravenous transplantation of heterogenous umbilical cord mesenchymal stem cells

Zhang Xue-feng, Zhang Mei-rong, Hu Jian-xia, Wang Li, Zhang Gui-zhi, Shao Lei, Li Sheng-gang, Gao Hong, Miao Zhi-min, Wang Yan-gang   

  1. Stem Cell Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao  266000, Shandong Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Wang Yan-gang, Doctor, Chief physician, Stem Cell Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, Shandong Province, China wangyg1966@ yahoo.com.cn
  • About author:Zhang Xue-feng★, Master, Physician, Stem Cell Center, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266000, Shandong Province, China xuefengzhang15@126.com

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Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells (HUMSCs) can be used as cells for tissue repair and regeneration engineering due to its abundant source, strong proliferation and differentiation ability, uninvolved to ethics problems. However, few studies have addressed the safety of heterogenous HUMSC transplantation via intravenous injection.
OBJECTIVE: To study the safety of intravenous injection of HUMSCs in Wistar rats.
METHODS: A total of 30 SPF Wistar rats were equally and randomly divided into three groups: blank control, negative control and HUMSC transplantation groups, of equal gender. HUMSCs were isolated and cultured in vitro. Rats in each group were injected via tail vein, with 1 mL of 5×106 HUMSCs as HUMSC transplantation group and 1 mL of 50 g/L glucose as negative control. The rats were observed for 14 days for their general reactions before they were sacrificed. Blood samples were collected for hematological and biochemical studies. Visceral organs were inspected, weighted and studied pathologically.
RESULTS AND CONCLUSION: No significant difference in hematological and biochemical studies was detected between HUMSC transplantation group and control group (P > 0.05). No significant difference was determined in histopathological observation in the viscera organs between HUMSC transplantation group and control group. Results have suggested that intravenous transplantation of heterogenous HUMSCs is safe and feasible. No side effects were found in recipients.

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