Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (32): 5931-5934.doi: 10.3969/j.issn.1673-8225.2010.32.009

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Mild hypothermia effects on migration of bone marrow mesenchymal stem cells in rats with cerebral ischemia 

You Hui1,Peng Yu1,Zhang Qi-mei1,Li Cheng-yan2   

  1. 1 First Clinical Medical College, Three Gorges University, Yichang  443003, Hubei Province, China; 2 Department of Neurology, Renmin Hospital, Wuhan University, Wuhan  430070, Hubei Province, China
  • Online:2010-08-06 Published:2010-08-06
  • Contact: Peng Yu, Master, Attending physician, First Clinical Medical College, Three Gorges University, Yichang 443003, Hubei Province, China ycpengu@126.com
  • About author:You Hui, Attending physician, First Clinical Medical College, Three Gorges University, Yichang 443003, Hubei Province, China

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Abstract:

BACKGROUND: There are a few reports addressing application of mild hypothermia to the study of repairing nerve injury, but few reports have addressed mild hypothermia effects on migration of neural stem cells in the brain following transplantation.
OBJECTIVE: To explore the effects of mild hypothermia on migration of BMSCs transplanted into lateral ventricle of rats after cerebral ischemia.
METHODS: A focal cerebral ischemic injury model of right middle cerebral artery occlusion (MCAO) was established using modified Longa’s method in Sprague Dawley rats. Local mild hypothermia was applied in the mild hypothermia group prior to transplantation. Normal body temperature was maintained in control group before transplantation. At 24 hours following model establishment, BMSCs labeled with 5-BrdU were transplanted into the rat lateral ventricle under brain stereotaxis. The amount of BrdU-positive cells was measured by immunohistochemistry at 5, 14, 21, 28 days and 7 weeks following injection in each group.
RESULTS AND CONCLUSION: At 14 days after transplantation, a majority of labeled BMSCs had migrated to the infarct area. At various time points following transplantation, the number of BrdU-positive cells was obviously greater in the hypothermia group compared with the control group (P < 0.05). Results have indicated that mild hypothermia may promote the migration of BMSCs, and shows neuroprotective effects on focal cerebral ischemia.

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