Abstract:

BACKGROUND: The directional induced differentiation of bone marrow stromal stem cells (BMSCs) into osteoblasts has disadvantages of high inducer price, difficult to prepare or long cell culture cycle and low osteogenic ability. Previous studies have confirmed that growth factor in concentrated platelet can induce osteanagenesis.
OBJECTIVE: To observe effects of platelet-rich plasma (PRP) on the proliferation and osteoblast differentiation activity of rabbit BMSCs cultured in vitro.
METHODS: Bone marrow was taken from the greater trochanter of bilateral femur in 8 rabbits. The rabbit BMSCs were separated and cultivated in vitro. The third generation of BMSCs was divided into two groups. In the experimental group, BMSCs were interfused with DMEM containing 1% PRP, while in the control group, BMSCs were incubated in common DMEM conditioned medium.
RESULTS AND CONCLUSION: Under an inverted microscope, the primary cells began to adhere to the wall at 24-36 hours. The cells were confluent at 10 -12 days. Cells in experimental group began to adhere on day 2. Most of the cells were confluent on day 6. The shape change of cells from the control group was later 1-3 days than that of the experimental group. At 2, 6, 10 and 14 days, alkaline phosphatase determination and the calcium node alizarin red staining showed that the rabbit osteobalasts cultured in vitro grew well by this method and the biochemical indexes were stable. They also had the same morphological and biological characters as the osteoblasts. These verified that the PRP can promote the BMSCs proliferation, and can also accelerate effectively the differentiation from BMSCs into osteoblasts.