Effect of human umbilical cord mesenchymal stem cells on acute lung injury in newborn rats

doi:10.3969/j.issn.2095-4344.2013.49.013

Abstract

Abstract:

BACKGROUND: A series of studies have found that mesenchymal stem cells play an important role in the prevention and cure of acute lung injury in adult animals.
OBJECTIVE: To further validate the effects of human umbilical cord mesenchymal stem cells on endotoxin-induced acute lung injury in newborn rats.
METHODS: Totally 120 newborn rats aged 7 days were randomly assigned to three groups. Intraperitoneal injection of 3 mg/kg endotoxin was done to establish neonatal rat model of acute lung injury in the model and stem cell group. Rats in the normal saline group were intraperitoneally injected with 0.1 mL normal saline. After 30 minutes of modeling, the rats in the stem cell group were subjected to intraperitoneal injection of 0.1 mL human umbilical cord mesenchymal stem cells (1×10⁶). The same volume of normal saline was administered in the normal saline and model groups. Lung tissue and blood specimens from newborn rats were taken at 6 hours, 1 day, 2 days, 4 days, and 7 days after treatment to observe lung pathological changes and detect levels of serum tumor necrosis factor-alpha and interleukin-10 as well as myeloperoxidase activity in the lung tissue.
RESULTS AND CONCLUSION: The lung hematoxylin-eosin staining and myeloperoxidase activity indicated acute lung injury in the model group. At 4 and 7 days after modeling, the severity of lung injury in the stem cell group was lighter than that in the model group. Compared with the model group, the interleukin-10 level was significantly increased in the stem cell group, while the level of tumor necrosis factor-alpha was significantly reduced (P < 0.05). These findings suggest that human umbilical cord mesenchymal stem cells transplanted into newborn rats with acute lung injury can reduce lung inflammation, and the main mechanism may be that human umbilical cord mesenchymal stem cells can balance anti-inflammatory and pro-inflammatory factors and reduce lung injury through immune regulation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, interleukin-10, tumor necrosis factor-alpha

CLC Number:

R394.2

Tu Hui-ying, Wu Ben-qing, Chen Li, He Wu-shi, Ding Lu, Huang Jin-jie. Effect of human umbilical cord mesenchymal stem cells on acute lung injury in newborn rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(49): 8545-8550.

Figures/Tables 4

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