Human placenta-derived mesenchymal stem cell transplantation promotes tendon graft healing in a bone tunnel

doi:10.3969/j.issn.2095-4344.2013.49.012

Abstract

Abstract:

BACKGROUND: Studies have shown that human placenta-derived mesenchymal stem cells with strong proliferative ability have rich sources and can remarkably promote tendon-bone healing after cell transplantation.
OBJECTIVE: To observe the effect of human placenta-derived mesenchymal stem cells on tendon-bone healing in a bone tunnel.
METHODS: Human placenta-derived mesenchymal stem cells were separated using adherent separation screening method. Thirty 8-week-old male Sprague-Dawley rats were randomly divided into two groups, 15 rats as experimental group and 15 rats as control group. Experimental group were subjected to transplantation of human placenta-derived mesenchymal stem cells and the control group were injected with saline solution.
RESULTS AND CONCLUSION: Stem cells, new vessels, and fibrocartilage hyperplasia were observed on the tendon-bone interface with microscope at 2, 4 and 6 weeks after cell transplantation in the experimental group. Biomechanically, the maximum pullout load in the experimental group was significantly higher than that in the control group at 4 and 6 weeks after cell transplantation (P < 0.05). These findings suggest that human placenta-derived mesenchymal stem cells can accelerate early tendon-bone healing in a bone tunnel and strengthen the biomechanical strength.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, tenotomy, cell transplantation

CLC Number:

R394.2

Yang Li-feng, Liu Wei, Zhou Ye, Feng Zi-shan, Zhang Li, Zhao Wei, Li Zhi . Human placenta-derived mesenchymal stem cell transplantation promotes tendon graft healing in a bone tunnel[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(49): 8539-8544.

Figures/Tables 5

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