Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3525-3527.doi: 10.3969/j.issn.1673-8225.2011.19.024

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Cryopreservation of hematopoietic stem cells with ladder-style freezing from low temperature refrigerator to liquid nitrogen method versus programmed cooling method

Zhao Ren-bin, Zhang Ai-ling, Lu Jie, Yang Ze-feng, Yang Tong-hua   

  1. Department of Hematology, First People’s Hospital of Yunnan Province, Kunhua Hospital of Kunming Medical University, Kunming  650032, Yunnan Province, China
  • Received:2011-02-10 Revised:2011-04-07 Online:2011-05-07 Published:2011-05-07
  • Contact: ang Tong-hua, Master’s supervisor, Chief physician, Department of Hematology, First People’s Hospital of Yunnan Province, Kunhua Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China ynanblood@yahoo.com.cn
  • About author:Zhao Ren-bin, Technician in charge, Department of Hematology, First People’s Hospital of Yunnan Province, Kunhua Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China zhaorenbin@126.com
  • Supported by:

    Specific Fund Program of Yunnan Science and Technology Committee & Kunming Medical University, No. 2007C00036R*

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Abstract:

BACKGROUND: During the cryopreservation of hematopoietic stem cells, there are many influential factors, such as cooling rate, storage temperature, and cryoprotectant combination. There is a controversy in the cryopreservation methods.
OBJECTIVE: To investigate the differences between -80 ℃ ladder-style freezing from low temperature refrigerator to liquid nitrogen method and programmed cooling method in preserving peripheral blood stem cells (PBSCs).
METHODS: Collected PBSCs were divided into two groups, and respectively cryopreserved by using -80 ℃ ladder-style freezing from low temperature refrigerator to liquid nitrogen method and programmed cooling method.
RESULTS AND CONCLUSION: There was no significant difference between these two frozen samples of PBSCs after recovery in the aspects of trypan blue exclusion rate, recovery, apoptosis rate and mortality (P > 0.05). These findings indicate that the -80 ℃ ladder-style freezing from low temperature refrigerator to liquid nitrogen method using the cryoprotectant containing 5% dimethyl sulfoxide, 4% albumin and 3% hydroxyethyl starch can yield the same effect as same as the programmed cooling method using 10% DMSO as cryoprotectant in the aspect of PBSCs cryopreservation, and the former one is more convenient and more suitable for clinical application.

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