Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3522-3524.doi: 10.3969/j.issn.1673-8225.2011.19.023

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Effect of pH levels in the survival and proliferation of adipose derived mesenchymal stem cells

Wu Kun1,2, Li Fang1, Ma Jian1,2, Li Jin-zhen1,2   

  1. 1Department of Orthopedics, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing  100700, China
    2Second Clinical Medical College, Shanxi Medical University, Taiyuan  030001, Shanxi Province, China
  • Received:2010-12-08 Revised:2011-04-09 Online:2011-05-07 Published:2011-05-07
  • Contact: Li Fang, Professor, Chief physician, Department of Orthopedics, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing 100700, China fang16722@vip.sina.com
  • About author:Wu Kun★, Studying for master’s degree, Physician, SDepartment of Orthopedics, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing 100700, China ; Second Clinical Medical College, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China koalaxxmc123@163.com

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Abstract:

BACKGROUND: Decreased pH value has a great effect on adipose derived mesenchymal stem cells (ADSCs) in the degenerated intervertebral disc.
OBJECTIVE: To observe the survival and proliferation of cultured ADSCs under different pH conditions.  
METHODS: ADSCs were isolated from fat tissue of rabbits and cultured under four different pH conditions (pH 7.4, pH 7.1, pH 6.8, pH 6.5). Five days later, typan blue staining was used to detect cell viability and MTT method was applied to draw cell growth curve.
RESULTS: The cell viability in the pH 6.5 group was the lowest compared with that in the other three groups (P < 0.05). It is indicated that pH 6.5 can inhibit the viability and proliferation of ADSCs.

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