Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3559-3562.doi: 10.3969/j.issn.1673-8225.2011.19.033

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Directed differentiation of ectomesenchymal cells isolated from the first branchial arch into adipocytes

Hou Jing-qiu1, 2, Yan Zheng-bin1, 2, Yang Xu2   

  1. 1Department of Stomatology, Daqing Oil Field General Hospital, Daqing  163001, Heilongjiang Province, China
    2College of Stomatology, Jiamusi University, Jiamusi  154004, Heilongjiang Province, China
  • Received:2010-11-02 Revised:2010-12-11 Online:2011-05-07 Published:2011-05-07
  • Contact: Yan Zheng-bin, Doctor, Associate chief physician, Department of Stomatology, Daqing Oil Field General Hospital, Daqing 163001, Heilongjiang Province, China; College of Stomatology, Jiamusi University, Jiamusi 154004, Heilongjiang Province, China yanzhengbin@163.com
  • About author:Hou Jing-qiu★, Master, Associate chief physician, Department of Stomatology, Daqing Oil Field General Hospital, Daqing 163001, Heilongjiang Province, China; College of Stomatology, Jiamusi University, Jiamusi 154004, Heilongjiang Province, China

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Abstract:

BACKGROUND: During the dentofacial development, the first branchial arch, as a transitional structure, plays an important role.
OBJECTIVE: To investigate the self-renewal of ectomesenchymal cells (EMCs) isolated from the first branchial arch as well as the feasibility of inducing EMCs into adipocytes.
METHODS: The activity of proliferation and self-renewal of EMCs were detected by nuclei labeled with BrdU and fluorescence in situ hybridization for telomerase. Monolayer cultures of EMCs were passaged 3 times and then transferred to adipogenic media. The level of differentiation was evaluated by histological examination and the expression of tissue-specific genes was analyzed by reverse transcription-polymerase chain reaction technique.
RESULTS AND CONCLUSION: Nuclei labeled with BrdU and fluorescence in situ hybridization for telomerase were positive. After being cultured in adipogenesis-inducing medium, EMCs responded by the accumulation of lipid vacuoles and the gene expression of LPL. The findings indicate that EMCs have a high activity of proliferation and self-renewal, and they are capable of differentiation into adipocytes in vitro.

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