Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3542-3545.doi: 10.3969/j.issn.1673-8225.2011.19.029

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Three-dimensional reconstruction and display of embryoid body-like structure

Shen Xiao-tao1,2,3, Zheng Xin1,2,3, Zhong Jia-yong4, Zhang Zhi-peng4, Li Zhen1,2,3, Cai Dong-qing1,2,3   

  1. 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, 4Department of Biomedical Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2011-01-19 Revised:2011-03-17 Online:2011-05-07 Published:2011-05-07
  • Contact: Cai Dong-qing, Professor, Doctoral supervisor, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, Jinan University, Guangzhou 510632, Guangdong Province, China tdongbme@jnu.edu.cn
  • About author:Shen Xiao-tao★, Master, Assistant technician, 1Key Laboratory for Regenerative Medicine, Ministry of Education, 2Joint Laboratory for Regenerative Medicine, the Chinese University of Hong Kong-Jinan University, 3the International Base of Collaboration for Science & Technology, the Ministry of Science and Technology & Guangdong Province Science and Technology Bureau, Jinan University, Guangzhou 510632, Guangdong Province, China tsxt@jnu.edu.cn
  • Supported by:

    the National “863” Program, No. 2007AA02Z105*; the National Natural Science Foundation of China, No. 30973158*, 30770886*, 30570369*, 30340038*; Guangdong Natural Science Foundation, No. 04105826*; Guangdong Science and Technology Development Program, No. 2004B30601007*; International Collaborated Program of Guangdong, No.  2009B050900007*; The Fundamental Research Funds for the Central Universities, No. 21609408*

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Abstract:

BACKGROUND: Stem cells have been widely studied and are considered as important cells for cell therapy since stem cells are capable of proliferation and differentiation. It is believed that three-dimensional (3D) reconstruction and display of stem cells and tissues is important to better understand the mechanisms of proliferation and differentiation of stem cells.
OBJECTIVE: To reveal and analyze embryoid body-like tissue structure using 3D-reconstruction technique.
METHODS: Differentiated embryoid body-like tissue structure was used as a model. Immunofluorescent staining combined with Z-axis consecutive image capture and 3D-reconstruction were used to reveal and analyze differentiated embryoid body-like cardiac myocyte tissue.
RESULTS AND CONCLUSION: Methods used in this study were able to reveal 3D-reconstruction of embryoid body-like tissue structure. Reconstructed 3D model could be rotated at any angle. After iso-surface processing under certain color channel, spatial relationship of cardiac troponin I positive tissue structure and nucleus could be displayed on related color channel or at any angle dimension. It was found that the nuclei were included in the differentiated embryoid body-like cardiac myocyte tissue. The results of present study documented that differentiated embryoid body-like cardiac myocyte tissue was able to reveal and analyze using the 3D-reconstruction technique.

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