Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3494-3498.doi: 10.3969/j.issn.1673-8225.2011.19.017

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Bone marrow mesenchymal stem cells prevent myocardial fibrosis via nuclear factor kappa B signaling pathway

Deng Wei1, Chen Qing-wei1, Li Xing-sheng1, Li Gui-qiong1, Ke Da-zhi1, Mo Xian-gang1, Zan Li-ping2   

  1. 1Department of Gerontology, Second Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
    2Department of Endocrinology, First Affiliated Hospital of Chongqing Medical University, Chongqing  400010, China
  • Received:2010-10-08 Revised:2010-11-13 Online:2011-05-07 Published:2011-05-07
  • Contact: Chen Qing-wei, Professor, Doctoral supervisor, Department of Gerontology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China chenqwcq@yahoo.com.cn
  • About author:Deng Wei☆, Studying for doctorate, Attending physician, Department of Gerontology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China dengwei1176@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30970826*

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Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) would show much help in treating myocardial fibrosis, and the mechanism involved remains unclear.
OBJECTIVE: To investigate the efficacy and mechanism of myocardial fibrosis intervention using BMSCs.
METHODS: BMSCs isolated from male mice were transfused into female mice with isoproterenol-induced myocardial fibrosis via tail vein (treated group). This study included three groups: treated, untreated, and control groups. Five weeks after transplantation, expression levels of sex-determining region of Y-chromosome (SRY), matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in myocardium were detected by fluorescent quantitative RT-PCR. Collagen distribution was observed using sirius red staining. The expression of nuclear factor-κB (NF-κB) was detected by immunohistochemistry.
RESULTS AND CONCLUSION: BMSCs can home to fibroid heart. Compared to the untreated group, treated group had decreased levels of MMP-9, TIMP-1, NF-κB and collagen deposition (P < 0.05). The results show that BMSCs transplantation can improve MMP-TIMP expression and collagen deposition in injured myocardium after homing, a mechanism that may be related to the NF-κB mediated signaling pathway.

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