Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (19): 3467-3471.doi: 10.3969/j.issn.1673-8225.2011.19.011

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Expression of nm23-H1 during differentiation of human umbilical cord mesenchymal stem cells into adipocytes and osteoblasts in vitro

Rong Jing1, Peng Xin-lei1, Ma Yan-yan1, Liu Qiu-ying1, Zhou Xiang-rong 2, Luo Xin3, Wang Yi-fei1   

  1. 1Guangzhou (Jinan) Biomedicine Research & Development Center, Guangzhou  510632, Guangdong Province, China
    2Asia Pacific Stem Cell Science Limited, Hong Kong, China
    3Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2011-01-24 Revised:2011-03-17 Online:2011-05-07 Published:2011-05-07
  • Contact: Wang Yi-fei, Professor, Guangzhou (Jinan) Biomedicine Research & Development Center, Guangzhou 510632, Guangdong Province, China twangyf@jnu.edu.cn
  • About author:Rong Jing★, Studying for master’s degree, Guangzhou (Jinan) Biomedicine Research & Development Center, Guangzhou 510632, Guangdong Province, China lydia033rj@qq.com
  • Supported by:

     International Cooperation Projects, No. 2008DFA30590*; Project of Guangzhou (Jinan)-Hong Kong Cell Engineering United Laboratory*; the National Natural Science Foundation of China, No. 81070459*; the Fundamental Research Funds for the Central Universities*

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Abstract:

BACKGROUND: It has been proved that nm23-H1 gene has a great infulence on proliferation, differentiation and transfering of many kinds of cells.
OBJECTIVE: To study on the expression of nm23-H1 gene during differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) into adipocytes and osteoblasts in vitro.
METHODS: The hUCMSCs were isolated from human umbilical cord by tissue adherence, and a flow cytometry was used for immunophenotype identification. Then the 3rd passage cells were induced to differentiate into adipocytes and osteoblasts, characterized by oil red O staining and Alizarin red staining respectively. The expression of nm23-H1 mRNA was detected by real time quantitative RT-PCR during the adipogenic and osteogenic differentiation.
RESULTS AND CONCLUSION: During the adipogenic differentiation, the nm23-H1 expression was increased in mRNA level after the induction on 4 days, and reached to the highest level on 7 days (P < 0.01), and then was gradually decreased on 14 days to the primary level which had no significant changes compared with untreated control (P > 0.05). During the osteogenic differentiation, the nm23-H1 expression in mRNA level was downregulated (P < 0.01) until maturation on 28 days (P > 0.05). nm23-H1 gene has shown a regulatory changes during the differerntiation of hUCMSCs into adipocytes and osteoblasts, this might imply that nm23-H1 gene make a contribution to the osteogenic and adipogenic differentiation.

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