Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3341-3346.doi: 10.3969/j.issn.1673-8225.2011.18.027

Previous Articles     Next Articles

Surgical technique improvement for liver transplantation in rats using 30% small-for-size grafts

Li Jiang1, Liu Jing2, Hou Yu3, Li Li2   

  1. 1Department of Hepatobiliary Surgery, First Affiliated Hospital of Kunming Medical University, Kunming  650032 , Yunnan Province, China
    2First Department of Hepatobiliary Surgery, Ganmeit Affiliated Hospital of Kunming Medical University, Kunming  650011, Yunnan Province, China
    3Department of Radiation Oncology, Third Affiliated Hospital of Kunming Medical University, Kunming  650118, Yunnan Province, China
  • Received:2010-11-25 Revised:2011-01-10 Online:2011-04-30 Published:2011-04-30
  • Contact: Li Li, Professor, Doctoral supervisor, First Department of Hepatobiliary Surgery, Ganmeit Affiliated Hospital of Kunming Medical University, Kunming 650011, Yunnan Province, China llkmchina@ yahoo.com.cn
  • About author:Li Jiang☆, Studying for doctorate, Department of Hepatobiliary Surgery, First Affiliated Hospital of Kunming Medical University, Kunming 650032 , Yunnan Province, China lijiangchina@ yahoo. com.cn
  • Supported by:

     the Major Program of Yuman Science and Technology Comittee Province, No.2007CA007*

PDF

448

Abstract:

BACKGROUND: The techniques of partial liver transplantation, using a living donor graft, expand the supply of organs and partially overcome the grafts shortage. But these benefits are limited in adult recipients when the volume of the grafts is small. To establish a model of small-for-size liver transplantation, using a simple and effective way, is the basis for the study mentioned above.
OBJECTIVE: To explore a simple and effective way of establishing a 30% small-for-size liver transplantation in rats.
METHODS: 280 Spraque-Dawley rats were selected as the donors and recipients to establish 30% small-for-size orthotropic live transplantation using two-cuff techniques. Animals were divided into two groups depending on the techniques modified or not. Sixty pairs of rats were divided into before modification group, using the way according to reference 10 to 15 and the median lobe of the liver as graft. These 60 pairs of rats were divided into two groups again depending on hepatectomy in vivo or in vitro. Group Ⅰ, performed hepatectomy in vivo before liver irrigation; groupⅡ, performed hepatectomy in vitro after liver irrigation. Eighty pairs of rats transplanted using the way of improvement by us were divided into modification group (group Ⅲ), in which hepatectomy was performed in suit after liver irrigation, the median and right lobes of liver were used as graft, body weights of donor were 100-120 g less than those of recipients, two-cuff technique and bile duct stent techniques were improved. Time of operation, survival and technical complications were compared among these groups.
RESULTS AND CONCLUSION: The time of hepatectomy and graft harvest were significantly shorter in group Ⅲ than those in group Ⅱ andⅠ(P < 0.05). The cold ischemia time in group Ⅱ was significantly longer than those in groupⅠand Ⅲ(P < 0.05). The incidence of bleeding, bile leakage IVC stricture, graft less perfusion and gas embolism were significantly less in group Ⅲ than those before modification. The rats in group Ⅲ had a higher transplanting successful rate, more 7-d and 14-d survivors post operation and longer median survival time (14 d) than those before modification (P < 0.05). The way of modification by us was a more effective and simple for establishing a 30% small-for-size liver transplantation in rats with higher transplanting successful rate and survival rate but fewer complications after operation.

CLC Number: