Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (18): 3259-3262.doi: 10.3969/j.issn.1673-8225.2011.18.008

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Clinical implication of monitoring interleukin-18 receptor in peripheral lymphocytes using flow cytometric after renal transplantation

Hao Jun-wen1, Min Zhi-lian2, Li Xiang-tie1, Chen Ying-jian1, Zheng Jun-hua2   

  1. 1Department of Urology, General Hospital of Jinan Military Area Command of Chinese PLA, Jinan  250031, Shandong Province, China
    2Department of Urology, Changzheng Hospital of Second Military Medical University, Shanghai  200003, China
  • Received:2010-11-17 Revised:2011-03-18 Online:2011-04-30 Published:2011-04-30
  • Contact: Min Zhi-lian, Professor, Department of Urology, Changzheng Hospital of Second Military Medical University, Shanghai 200003, China
  • About author:Hao Jun-wen☆, Doctor, Chief physician, Department of Urology, General Hospital of Jinan Military Area Command of Chinese PLA, Jinan 250031, Shandong Province, China hjw.jnjz@126.com
  • Supported by:

    the Key Subject of Military Medical Research during the Tenth Five-year Period, No. 01Z061*

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Abstract:

BACKGROUND: As cytokines are often released in the early stage following renal transplantation, they can used as an indicator for early diagnosis of rejection.
OBJECTIVE: To investigate the expression of interleukin 18 receptor (IL-18R) in peripheral blood of uremia patients before and after renal transplantation.
METHODS: The expression of CD4/IL-18Rα and CD8/IL-18Rα in peripheral lymphocytes was determined using flow cytometric analysis in 32 uremia patients receiving renal transplantation including 24 patients with stable function, 8 with acute rejection, 12 who had a long-term survival (average 4 years), and 7 healthy controls.
RESULTS AND CONCLUSION: CD4/IL-18Rα and CD8/IL-18Rα positive rates were higher in the uremia patients than the healthy controls (P=0.02, P=0.04). The expression of CD4/IL-18Rα and CD8/IL-18Rα in peripheral lymphocytes in the patients with stable function and those who had a long-term survival was lower than that in the patients with acute rejection. After steroid plus therapy, the positive rates for CD4/IL-18Rα and CD8/IL-18Rα were decreased significantly in the patients with acute rejection (P < 0.05). IL-18Rα expression was stronger after steroid pulse therapy in the 3 patients resistant to steroids than in the 5 patients responsive to steroid treatment. The results demonstrate that detection of CD4/IL-18Rα and CD8/IL-18Rα in the peripheral lymphocytes by flow cytometric analysis was an easy, hopeful, and noninvasive means for the diagnosis and treatment of patients with acute rejection after renal transplantation.

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