Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6776-6779.doi: 10.3969/j.issn.1673-8225.2010.36.028

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Immunosuppressive action of human placenta mesenchymal stem cells in mixed lymphocyte reaction system  

Xue Yi-feng1, Huang Jian-rong1, Sun Hong-li1, Miao Zong-ning2   

  1. 1 Department of Laboratory Medicine, Wuxi People’s Hospital, Wuxi  214023, Jiangsu Province, China; 2 Key Laboratory of Stem Cell Research of Jiangsu Province, Wuxi  214041, Jiangsu Province, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Miao Zong-ning, Doctor, Technician-in-charge, Key Laboratory of Stem Cell Research ofJiangsu Province, Wuxi 214041, Jiangsu Province, China zongningm@163.com
  • About author:Xue Yi-feng, Technician-in-charge, Department of Laboratory Medicine, Wuxi People’s Hospital, Wuxi 214023, Jiangsu Province, China yf.xue@163.com

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Abstract:

BACKGROUND: Placenta comprises trophoblasts, blood vessels and plenty of mesenchyme that derived from the mesoderm. Thus, there are mesenchymal stem cells (MSCs) in placenta. 
OBJECTIVE: To explore the effect of placenta MSCs (PMSCs) on the proliferation of T lymphocytes and secretion of cytokines in the mixed lymphocyte reaction system, and to evaluate the immunological characteristics of PMSCs.
METHODS: The two-way mixed lymphocyte reaction (MLR) system both for human and rabbits were established. After adding PMSCs which was treated by the mitomycin with different ratios (1:5, 1:10, 1:20, 1:50, 1:100, 1:500) in the MLR system, the lymphocyte proliferation rate were measured by 3H incorporation, and the content of interleukin-2 and interferon-γ in the supernatant was detected by ELISA.
RESULTS AND CONCLUSION: The proliferation of T lymphocytes could be inhibited to different extents by adding different ratios of PMSCs to human or rabbit PBMCs, and the inhibition rate increased as the ratio of PMSCs to PBMCs increased. Meanwhile, PMSCs could reduce cytokines interleukin-2 and interferon-γ secretion in the mixed lymphocyte reaction system. The PMSCs have the immune regulation ability and inhibit xenogeneic immune cells.

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