Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6729-6732.doi: 10.3969/j.issn.1673-8225.2010.36.018

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Allogeneic bone marrow mesenchymal stem cells transplantation for spinal cord injury in rats

Ruan Zhi, Huang Hui, Sun Jian-hua, Shi Chen-hui, Dong Jin-bo, Wang Yong-ming   

  1. First Department of Orthopedics, First Affiliated Hospital, Medical College, Shihezi University, Shihezi  832008, Xinjiang Uygur Autonomous Region, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Shi Chen-hui, Master, Chief physician, Professor, First Department of Orthopedics, First Affiliated Hospital, Medical College, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China sch7890@yahoo. com.cn
  • About author:Ruan Zhi★, Studying for master’s degree, Attending physician, First Department of Orthopedics, First Affiliated Hospital, Medical College, Shihezi University, Shihezi 832008, Xinjiang Uygur Autonomous Region, China ruanzhi113@sina.com

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Abstract:

BACKGROUND: There is no good therapeutic tool for repairing spinal cord injury (SCI). Cell transplantation can contribute to axonal regeneration and the recovery of spinal cord function, and provide possibility for treating SCI. However, the SCI models and transplanted manners are various, and the therapeutic efficiency is different.
OBJECTIVE: To investigate the therapeutic effects of allogeneic bone marrow mesenchymal stem cells (BMSCs) transplantation on rat SCI.
METHODS: BMSCs were isolated from rats by the whole bone marrow method. Healthy Sprague Dawley rats were randomly assigned to cell transplantation, control and sham surgery groups. In the cell transplantation and control groups, rat SCI models were established by the modified Allen’s method. In the sham surgery group, the spinal cord was exposed only. At 4 weeks following surgery, locomotive function of SCI rats was graded weekly. Enzyme linked immunosorbent assay was used to detect expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in injured spinal cord. Immunofluorescent staining was employed to detect the expression of NF200 and glial fibrillary acidic protein (GFAP). 
RESULTS AND CONCLUSION: Compared with control group, movement function was significantly improved, and BDNF and NGF protein contents were significantly increased in the spinal cord in the cell transplantation group (P < 0.05). The spinal cord capsular space was small; NF200 expression was significantly increased; GFAP expression was reduced in the rats from the transplantation group. Results indicate that allogeneic BMSCs transplantation could increase NGF levels, inhibit the glial scar formation and promote axonal regeneration and improve functional recovery in rats after SCI.

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