Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (36): 6685-6688.doi: 10.3969/j.issn.1673-8225.2010.36.008

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Biological characteristics and phenotypes of adipose tissue-derived stem cells

Cai Zhen1, Pan Bo2, Lin Lin2, Jiang Hai-yue2, Zhuang Hong-xing2   

  1. 1 Department of Plastic Surgery, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, Chengdu  610072, Sichuan Province, China; 2 Seventh Department of Plastic Surgery, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing  100144, China
  • Online:2010-09-03 Published:2010-09-03
  • Contact: Jiang Hai-yue, Professor, Doctoral supervisor, Seventh Department of Plastic Surgery, Plastic Surgery Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100144, China jianghaiyue@sohu. com
  • About author:Cai Zhen☆, Doctor, Attending physician, Department of Plastic Surgery, Sichuan Academy of Medical Sciences, Sichuan Provincial People’s Hospital, Chengdu 610072, Sichuan Province, China caizhen1976@126. com

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Abstract:

BACKGROUND: Adipose-derived stem cells (ADSCs) are capable of multi-directional differentiation and exist in human processed lipoaspirate. However, no in-depth studies have addressed biological characteristics of adipose tissue-derived stem cells (ADSCs) in vitro, and there are some disputes on the results from phenotype studies.
OBJECTIVE: To investigate biological characteristics and phenotypes of human ADSCs.
METHODS: Human adipose tissue was collected from persons undergoing fat extraction. The ADSCs were digested with collagenase, cultured and subcultured in vitro. Cell morphology was observed and growth curves were drawn. The phenotypes of ADSCs were identified by immunohistochemistry and flow cytometry.
RESULTS AND CONCLUSION: ADSCs grew well, presented fibroblast-like growth. No significant change in cell morphology was detected within passage 15. The results from immunohistochemistry showed that cells were positive for CD29, CD44, CD105, but negative for CD34, CD45. Flow cytometry results have shown that cells were positive for CD29, CD44, CD105, MHC-Ⅰ, but negative for CD3, CD14, CD19, CD31, CD33, CD34, CD45, CD106, CD117, CD184. Results have suggested that ADSCs can be abundantly harvested and have stable proliferation in poorly differentiated status in vitro. Stem cell-related antigens were highly expressed in ADSCs.

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