Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 5058-5061.doi: 10.3969/j.issn.1673-8225.2010.27.027

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Effects of lycium barbarum polysaccharides on proliferation and differentiation of rat bone marrow mesenchymal stem cells into cells of endothelial lineage  

Lin Zhuang-lin, Lu Wen-yue, Chen Ping, Zheng Hui-zhen   

  1. Guangdong Medical Collehanjiang  524023, Guangdong Province, China
  • Online:2010-07-02 Published:2010-07-02
  • Contact: Zheng Hui-zhen, Doctor, Professor, Master’s supervisor, Department of Physiology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China zhenghz6@gdmc.edu.cn
  • About author:Lin Zhuang-lin, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
  • Supported by:

    the Kangbo Innovation Foundation of Guangdong Medical College, No. 200801*; the Technology Plan Tender Project of Zhanjiang City, No. 2006-3-3*; the Natural Science Foundation of Guangdong Province, No. 9152402301000028*

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Abstract:

BACKGROUND: Lycium barbarum polysaccharides (LBP) can promote spermatogenic cell proliferation and induce the differentiation of rat bone marrow messenchymal stem cells (BMSCs) and human umbilical cord blood messenchymal stem cells into neuron-like cells. But, it is unknown that the effects of LBP on biological characteristics of BMSCs, which deserves further study.
OBJECTIVE: To investigate the effects of LBP on proliferation and endothelial differentiation of rat BMSCs.
METHODS: BMSCs were separated from Sprague Dawley rats by gradient centrifugation. Cell proliferation rate was analyzed by MTT assay. Rat BMSCs were induced to differentiate into endothelial lineage cells by HG-DMEN plus EGM-2. These cells were identified as endothelial cells with positive factor Ⅷ and Ulex europaeus agglutinin expression. The effects of LBP on proliferation and differentiation of rat BMSCs into endothelial lineage cells were analyzed by adding LBP or not in the medium.
RESULTS AND CONCLUSION: LBP in medium did not change the cellular morphology and did not affect the expression of cell surface marker CD29, CD45 or CD106 and with negative factor Ⅷ. However, LBP speeded up the proliferation of rat BMSCs and delayed its peak proliferation time. When rat BMSCs were cultured in endothelial induction medium containing EGM-2 or endothelial induction medium + LBP. In the induction group without LBP, rat BMSCs had changed into endothelial-like cells after 10 days of induction. However, these induced by medium containing LBP should change into endothelial-like cells by 12 days. These indicated that LBP can delay the differentiation of EGM-2-induced rat BMSCs into endothelial lineage cells.

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