Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 5023-5027.doi: 10.3969/j.issn.1673-8225.2010.27.019

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Growth and proliferation of bone marrow mesenchymal stem cells through in vitro culture of Zinc, basic fibroblast growth factor alone or their combination  

Ren Li-jie, Wang Yang, Han Man-fu   

  1. Department of Neurology, Shenzhen Second People’s Hospital, Shenzhen  518035, Guangdong Province, China
  • Online:2010-07-02 Published:2010-07-02
  • About author:Ren Li-jie☆, Doctor, Associate chief physician, Department of Neurology, Shenzhen Second People’s Hospital, Shenzhen 518035, Guangdong Province, China jesser2005@126.com
  • Supported by:

    the Scientific Research Foundation for Establishing Traditional Chinese Medicine Research of Guangdong Province, No. 2009319*

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Abstract:

BACKGROUND: It is the hot spot of neuroscience research addressing how to amplify a great quantity of bone marrow mesenchymal stem cells (BMSCs) in vitro by a convenient and effective way.
OBJECTIVE: To study the effects of Zinc and basic fibroblast growth factor (bFGF) alone or in combination on the growth and proliferation of BMSCs in vitro, and to search an effective method of rapidly amplifying a great quantity of BMSCs.
METHODS: Rat BMSCs were cultured in vitro. This study was composed of control group (without any influential factor), Zinc group, bFGF group, Zinc + bFGF groups, which was respectively treated with Zinc and bFGF alone or in combination. Cell growth curve was observed. Cell survival and proliferation were observed using MTT assay. Cell cycle was detected using flow cytometer, and cell surface antigen was identified.
RESULTS AND CONCLUSION: Cell growth curve, MTT assay and flow cytometer results have shown that cell reproductive activity reached a peak at 3-5 days, and Zinc + bFGF group showed the strongest ability (P < 0.05). At day 7, cell reproductive activity was decreased in the control group, bFGF group and Zinc group, and only cells in the Zine + bFGF group exhibited a strong reproductive activity (P < 0.05). The percentage of cells in S+G2+M phase was greatest in the Zinc + bFGF group, and significant differences were found as compared with other three groups (P < 0.01). Results have verified that Zinc and bFGF can contribute to BMSC proliferation in vitro. Combination of Zinc and bFGF exhibits strongest effects on promoting proliferation, and can be an optimal culture condition for BMSCs in vitro.

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