Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (27): 5015-5017.doi: 10.3969/j.issn.1673-8225.2010.27.017

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Application of frozen single platelet transfusion in peripheral blood stem cell transplantation: Comparison with fresh single platelet transfusion

Yang Xiao-shun1, Xie Lin2, Zhu Hong-jie3, Li Lin-hua1, Wang Gui-hua4   

  1. 1 Health Center, Yunnan Provincial Committee Organizations, Kunming  650032, Yunnan Province, China;2 Chemotheray Center, Yunnan Provincial Cancer Hospital, Kunming  650118, Yunnan Province, China; 3 Department of Emergency, 533 Hospital of Chinese  PLA, Kunming  650224, Yunnan Province, China; 4 Department of Blood Center, Kunming General Hospital of Chengdu Military Area Command of Chinese PLA, Kunming  650032, Yunnan Province, China
  • Online:2010-07-02 Published:2010-07-02
  • About author:Yang Xiao-shun, Associate chief technician, Health Center, Yunnan Provincial Committee Organizations, Kunming 650032, Yunnan Province, China

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Abstract:

BACKGROUND: Because voluntary blood donation in China leads to blood shortage, a large number of fresh single platelets are sometimes difficult to guarantee. Frozen single platelets have immediate hemostatic effect for patients who are in the low state platelets with bleeding.
OBJECTIVE: To study the effect of frozen single platelet transfusion on peripheral blood stem cell transplantation.
METHODS: A total of 44 patients with hematopathy or lymphoma were subjected to peripheral blood stem cell transplantation, and platelet was less than 40×109 /L. These patients were randomly assigned to frozen single platelets group and fresh single platelets group (n = 22). At 0-14 days following transplantation, patients in the fresh single platelets group were directly infused with fresh single platelets following leukocyte filtration. Homotype frozen single platelets were obtained from patients in the frozen single platelets group, and then placed in a water bath at 37 ?C for rapid thawing. Following filtration using leukocyte filter, infusion was conducted within 40 minutes; once every 3 days,  10 U/time, for totally 3-16 times. The time of infusion depended on bleeding of patients.
RESULTS AND CONCLUSION: No significant difference in platelet and four items of blood coagulation was detected between fresh single platelets group and frozen single platelets group at 24 hours following infusion (P > 0.05). Blood platelet count was significantly decreased at 48 hours following infusion (P < 0.01). Bleeding time, prothrombin time, activated partial thromboplastin time and platelet count were significantly reduced at 72 hours following infusion ( P < 0.05 or P < 0.01). There were no significant differences in thrombin time and fibrinogen (P > 0.05). It is suggested that the time of frozen single platelet transfusion to prevent bleeding in peripheral blood stem cell transplantation should change into every 2 days.

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