Abstract:

BACKGROUND: At present, temperature of recovery of bone marrow mesenchymal stem cells (BMSCs) is 37 ℃. Previous reports have confirmed that spermatogonia were recovered at a high temperature, but it remains poor understood whether BMSCs were recovered at a high temperature.
OBJECTIVE: To observe the effects of high temperature on recovery rate and differentiation ability of BMSCs.
METHODS: Rat BMSCs were isolated and cultured by adherence method. The fifth passage of BMSCs were cryopreserved, and then recovered at 37 ℃ and 50 ℃ in water bath. The recovery rate was compared using trypan blue staining in both groups. BMSCs were induced to differentiate into neuron-like cells by fibroblast growth factor + epidermal growth factor + ratinoic acid at 50 ℃ in water bath, and identified by immunohistochemical staining.
RESULTS AND CONCLUSION: Recovery rates were respectively (82.40±0.86)% and (87.10±1.08)% in the 37 ℃ and 50 ℃ groups (P < 0.01). In the 50 ℃ group, cells presented neuron-like changes following inducer intervention, and were positive for Nestin in immunohistochemical staining. Results have suggested that the recovery rate in BMSCs was higher in 50 ℃ group compared with 37 ℃ group. Moreover, the differentiation ability was not affected.