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    01 January 2026, Volume 30 Issue 在线 Previous Issue    Next Issue
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    Preliminary study on the mechanisms and efficacy of deep brain stimulation in treating depression
    Haonan Yang, Zhengwei Yuan, Junpeng Xu, Zhiqi Mao, Jianning Zhang
    2026, 30 (在线):  1-9. 
    Abstract ( 91 )   PDF (2598KB) ( 77 )   Save
    BACKGROUND: This study combined fiber photometry calcium signal recording with C-fos immunohistochemistry to systematically investigate the neural modulation mechanisms of deep brain stimulation (DBS) in the anterior cingulate cortex (ACC). It also compared the effects of DBS and electro-ablation on depressive-like behaviors, providing experimental evidence for optimizing neuromodulation treatment strategies. 
    METHODS: Grouping and Modeling: Mice were randomly divided into control, CRS_Sham (sham stimulation), and CRS_DBS (intervention) groups. The latter two groups underwent 3.5 weeks of chronic restraint stress (CRS) modeling and had DBS electrodes implanted in the ACC. Intervention Protocol: The DBS group received daily high-frequency electrical stimulation (130 Hz, 200 μA, 50 μs, 2 hours/day for 1 week), while the Sham group underwent electrode implantation without current stimulation. Behavioral Assessment: Depressive-like behaviors were quantified using the forced swim test (FST) and tail suspension test (TST). Anxiety-like behaviors were assessed via the open field test (OFT) in 10 mice (5 in each of the DBS and Sham groups). Circuit Mechanism Analysis: Real-time neural activity was monitored by fiber photometry to assess the activation effect of ACC-DBS on the ACC-BLA circuit. Whole-brain activation patterns were analyzed using C-fos immunohistochemistry to quantify downstream neuronal activity. Electro-ablation Validation: An additional experiment was conducted with an electro-ablation group and a sham stimulation group to verify their effects on depressive-like behaviors. 
    RESULTS: Behavioral Analysis: FST showed that immobility times in the CRS_DBS and CRS_Sham groups were significantly higher than in the control group (P < 0.05), but no significant difference was observed between the two groups. No significant differences were found in TST among the groups. OFT results indicated that central zone duration and total distance traveled were significantly lower in the DBS group than in the Sham group (P < 0.05), suggesting that DBS may exacerbate anxiety-like behaviors. In contrast, the immobility time in the electro-ablation group was significantly lower than in the sham stimulation group (P < 0.01). Neural Mechanisms: Fiber photometry confirmed that ACC-DBS specifically activated the ACC-BLA circuit. C-fos staining revealed that DBS significantly enhanced neuronal activity in downstream brain regions of the ACC.
    CONCLUSION: Traditional high-frequency ACC-DBS effectively activates target neural circuits but fails to improve depressive-like behaviors and may exacerbate anxiety states by enhancing limbic system activity. In contrast, ACC functional inhibition via electro-ablation demonstrates significant antidepressant effects, offering new directions for optimizing neuromodulation strategies.
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    Research context and trend of TBK1 in autoimmunity, signaling pathways, gene expression, tumor prevention and treatment
    Xu Canli, He Wenxing, Wang Yuping, Ba Yinying, Chi Li, Wang Wenjuan, Wang Jiajia
    2026, 30 (在线):  1-11. 
    Abstract ( 68 )   PDF (4796KB) ( 7 )   Save
    Abstract
    BACKGROUND: To explore the research status, hot spots, and trends of TBK1 based on bibliometric analysis and provide references and suggestions for the further basic research of TBK1. 
    Method: We collected TBK1 articles for the last 10 years based on the SCIE database in the Web of Science Core Collection. The data were imported into CiteSpace 6.3.R1 and analyzed bibliometrically and visually with five options: Country, Author, Institution, Reference, and Keyword, respectively. In addition, we used Origin 2021 to plot the relevant statistical graphs. 
    Result: There was an upward trend in the number of publications and co-citations involved in TBK1 research. Dan-Dan Chen, Jian-Fang Gui, Qiwei Qin, and Shun Li were the four authors with the highest number of publications (11), while the Chinese Academy of Sciences, University of Chinese Academy of Sciences, Zhejiang University, Chinese Academy of Agricultural Sciences, and Wuhan University had a larger number of publications (> 50). The research hot spots of TBK1 in the last decade mainly focused on innate immunity, the cGAS-STING pathway, NF-κB, inflammation, optineurin, expression, cancer, etc. Conclusion  In recent years, academics from various countries conducted sustained and in-depth research in related fields. TBK1 showed great potential for research in the autoimmune system, signaling pathways, gene expression, tumor control, and so on. But academic cooperation between academics and between institutions was not strong. In the future, cooperation and communication should be strengthened to grasp the research hot spots and trends of TBK1, to expand its research range in disease areas, and to provide more evidence for further elucidating the pharmacodynamic mechanisms and pathological changes of diseases
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    Neuromechanism of the endocannabinoid system in regulating exercise motivation
    Zhang Qingtong, Chen Leqin, Liu Chang, Chen Yuting, Guo Ruiwu
    2026, 30 (在线):  1-11. 
    Abstract ( 25 )   PDF (1964KB) ( 6 )   Save
    Abstract
    BACKGROUND: Recent studies have shown that the endocannabinoid system has a positive effect on the release of dopamine in the limbic cortex, and the reward mechanism of dopamine is a key factor influencing exercise motivation.
    OBJECTIVE: To systematically investigate the composition of the endocannabinoid system and its important role in neurotransmitter regulation, emotion regulation and pain perception, and to focus on exploring how the endocannabinoid system promotes the improvement of exercise motivation through the dopamine reward system, signal integration in motor control regions and fatigue recovery mechanism.
    METHODS: Computerized searches were conducted in CNKI, WanFang, VIP, PubMed, MedReading and Web of Science databases The search terms included “endocannabinoid system, exercise motivation, dopamine reward system, neuromodulation, cannabinoid receptor, anandamide, 2-arachidonoylglycerol” in Chinese and English. According to the inclusion and exclusion criteria, 84 articles were finally included for review.
    RESULTS AND CONCLUSION: This study reveals the significant role of the endocannabinoid system in promoting exercise motivation. (1) In the reward pathway, the endocannabinoid system acts on the mesolimbic dopamine pathway and stimulates dopamine release through downstream cannabinoid type 1 receptors, thereby enhancing the rewarding effect of the dopaminergic pathway and ultimately increasing the pleasure during exercise. (2) In terms of motor control, the endocannabinoid system achieves an optimal state by regulating synaptic plasticity changes in the ventromedial prefrontal cortex, and it precisely transmits signals to the striato-cortical circuit through spatial positioning to maintain the stability of exercise motivation. Moreover, the anti-inflammatory mechanism mediated by cannabinoid type 2 receptors can protect the nervous system from damage by inhibiting the excessive activation of microglial M1 polarization. (3) In terms of fatigue recovery, the endocannabinoid system, on the one hand, alleviates post-exercise discomfort and reduces fatigue caused by exercise by inhibiting the body’s pain receptors through cannabinoid type 1 or type 2 receptors. On the other hand, it can increase the plasticity of brain neurons and improve the structure of muscle fibers through the action of anandamide-brain-derived neurotrophic factor to help restore physical strength quickly. In conclusion, targeting the endocannabinoid system can effectively enhance people’s desire to engage in sports activities, , which has important clinical value and significance, especially for those who have not participated in physical exercise for a long time or those suffering from depression. However, more experimental evidence is needed to support this conclusion.

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