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    10 June 2015, Volume 19 Issue 在线 Previous Issue    Next Issue
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    Down-regulation of miR-221/222 inhibits cell proliferation and promotes chondrogenic differentiation of human bone marrow mesenchymal stem cells
    Yan Ji-hong, Yang Shu, Sun Hai-mei, Cao Dan-dan, Zhang Xiu-ying, Ji Feng-qing, Guo Duo, Wu Bo,
    2015, 19 (在线):  1-6. 
    Abstract ( 213 )   PDF (1836KB) ( 453 )   Save

    BACKGROUND: The use of mesenchymal stem cells in the field of tissue engineering for osteoarticular injury repair is a very promising tool since these cells are readily expandable and able to differentiate into chondrocytes. Abundant evidence suggests that miRNAs play critical roles in chondrogenic differentiation of mesenchymal stem cells.
    OBJECTIVE: To observe the chondrogenic effect of human bone marrow mesenchymal stem cells transfected with lentiviral vectors bearing miR-221-3p/222-3p inhibition, thereby provding new strategies for cartilage injury.
    METHODS: miRNA microarray technology was applied to detect miRNAs expression profiles at three different stages of chondrogenic differentiation induction after transforming growth factor-β3 treatment and verified by real-time fluorescence quantitative PCR (RT-qPCR). Human bone marrow mesenchymal stem cells were infected with lentivirus bearing miR-221-3p/222-3p inhibition. After co-suppressing the expression of miR-221/222-3p, cell counting kit-8 was used to determine the cell proliferation, the differentiation of bone marrow mesenchymal stem cells towards chondrocytes was verified by type II collagen protein expression through immunohistochemistry and glycosaminoglycan accumulation was also elevated by sarranine O staining. RT-PCR was used to detect type II collagen and aggrecan mRNA expression at 21 days of chondrogenic induction.
    RESULTS AND CONCLUSION: The expression of miR-221-3p/222-3p was inhibited after Lv-miR221-3p/222-3p inhibition co-transfected into bone marrow mesenchymal stem cells. miRNA microarray and RT-qPCR results showed that the expression of miR-221-3p/222-3p was declined significantly at the anaphase of chondrogenic differentiation. The expression levels of chondrogenic markers, Aggrecan and Collagen type II were significantly increased in the miR-221-3p/222-3p inhibition group and cell proliferation was also inhibited significantly compared with non-transduced cells or transduced with the empty lentiviral vector group. miR-221-3p/222-3p knockdown in bone marrow mesenchymal stem cells could inhibit proliferation but promote chondrogenic differentiation of bone marrow mesenchymal stem cells.

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    Efficacy and safety of posterolateral knee inverted “L” shaped pathway when placing the supporting plate
    Song Chun-jian, Sun Hui, Yang Guang, Shi Hui-peng
    2015, 19 (在线):  8585-8590.  doi: 10.3969/j.issn.2095-4344.2015.53.010
    Abstract ( 256 )   PDF (839KB) ( 377 )   Save

    BACKGROUND: Studies have shown that tibial plateau posterolateral fractures can be treated by posterolateral knee approach, however, there is no clear anatomical research to deeply evaluate and analyze this approach.
    OBJECTIVE: To evaluate the efficacy and safety of posterolateral knee inverted “L” shaped pathway in invloving tibial plateau posterolateral fractures.
    METHODS: Eight fresh frozen adult corpses, 16 knees side, were all dissected using posterolateral inverted “L” shaped pathway. During the dissection, the exposure range was observed and important parameters of anatomical structure were measured.
    RESULTS AND CONCLUSION: The pathway may be fully exposed to the posterolateral aspect of tibial plateau and posterior cruciate ligament tibial insertions. The operations completed by out team did not create any obvious interference to superior tibiofibular joint, fibular head and posterolateral corner structure. The exposed mean length of common peroneal nerve in incision was 56.48 mm, with a mean angle of 14.7° tilt towards the axis of the fibula. The mean distance between the neck of the fibular and fibular head tip was 31.26 mm, an average of
    42.18 mm to the joint line. The mean distance between the opening of the interosseous membrane and the 
    articular surface was 48.78 mm. The divergence of the fibular artery from the posterior tibial artery was an average of 76.46 mm from articular surface. These results confirm that posterolateral inverted “L” shaped pathway met the requirements of anatomical reduction and buttress fixation for posterolateral tibial plateau fracture. Exposure of the  common peroneal nerve can be minimized or even avoided by modifying the skin incision. Because the popliteal artery branches anterior tibial artery passed through interosseous membrane hole and peroneal artery and then separated from the posterior tibial artery, pathways dissection to distal deep area should be carried out carefully. Placement of a posterior buttressing plate carries a high vascular risk if the plate is implanted beneath these vessels.
    中国组织工程研究杂志出版内容重点:人工关节;骨植入物;脊柱骨折;内固定;数字化骨科;组织工程

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