Abstract:

BACKGROUND: The silk fibroin material has good biocompatibility.
OBJECTIVE: To observe the feasibility of silk fibroin material and bone marrow mesenchymal stem cells for repairing the rat full-thickness cutaneous defects.
METHODS: The bone marrow mesenchymal stem cells of Sprague-Dawley rats were labeled with 5-bromodeoxyuridine and cultured with silk fibroin material. The Sprague-Dawley rat full-thickness cutaneous defect models were prepared and divided into four groups: the experimental group was transplanted with the complex of allogeneic bone marrow mesenchymal stem cells and silk fibroin material; the cell group was transplanted with bone marrow mesenchymal stem cells; the control group was transplanted with silk fibroin material; and the blank group without any treatment.
RESULTS AND CONCLUSION: ①General observation: the necrotic tissue was observed in the blank group at 8 weeks after operation and the scar contracture was obvious; the wound healing in the cell group was similar with that in the blank group. The wound in the control group was not healed and a little amount of scar was observed and integrated with the surrounding skin. There was no obvious scar in the experimental group at 4 weeks after operation and the wound was healed well at 8 weeks after operation. ②Histological observation: the immunofluorescence staining in the experimental group at 4 weeks after operation showed that the 5-bromodeoxyuridine labeled bone marrow mesenchymal stem cells were located in reconstructed epidermis and dermal tissues, and wound surface was recovered well at 8 weeks after operation; the immunofluorescence staining in the cell group showed a little amount of 5-bromodeoxyuridine labeled bone marrow mesenchymal stem cells. It indicates that co-transplantation of silk fibroin material and bone marrow mesenchymal stem cells can repair rat full-thickness cutaneous defects.