Neuroprotective effects of hydrogen sulfide in rats with acute cauda equina syndrome

doi:10.3969/j.issn.2095-4344.2014.49.008

Abstract

Abstract:

BACKGROUND: Endogenous hydrogen sulfide can be used as a new gaseous signaling molecule, and has important signal transfer function and biological regulation effects.

OBJECTIVE: To study the neuroprotective effects of hydrogen sulfide in rats with acute cauda equina syndrome.

METHODS: The 72 Sprague-Dawley rats were randomly assigned to three groups. Experimental group, model group: laminectomy was performed at the lumbar 4 (L₄) level of the vertebra, and a piece of silicone (10 mm long, 1 mm thick, and 1 mm wide) was placed under the laminae of the L₅_-₆ vertebra to produce the animal model of cauda equina syndrome. Sham surgery group: a simple laminectomy was performed in L₄, but silicone was not implanted. In the experimental group, 20 μmol/kg NaHS was injected intraperitoneally at 1 hour before model establishment. Model and sham surgery groups: an equal volume of saline was injected intraperitoneally. At 12, 24, 48 and 72 hours after model establishment, malonaldehyde and glutathione levels in cauda equina nerve tissue were detected. Simultaneously, hematoxylin-eosin staining and TUNEL staining were performed at 48 hours.

RESULTS AND CONCLUSION: Hematoxylin-eosin staining demonstrated that cauda equina nerve tissue was dense and regular, with complete myelin sheath, no axon swelling in the sham surgery group. Cauda equina nerve tissue was sparse, with the presence of demyelination, and partial axons and myelin sheath swelling in the model group. Cauda equina nerve tissue was tight, with axonal swelling and demyelination in the experimental group. TUNEL staining demonstrated that the number of positive cells was less in the spinal cord and dorsal root ganglia in the sham surgery group. Abundant positive cells were detected in the spinal cord and dorsal root ganglia in the model group. The number of positive cells was significantly lower in the experimental group than that in the model group. Malonaldehyde levels were lower in the sham surgery and experimental groups than in the model group (P < 0.05, P < 0.01), but glutathione levels were higher than model group (P < 0.05, P < 0.01). These results indicated that hydrogen sulfide could decrease oxidative stress and protect cauda equina nerve in rats with acute cauda equina syndrome.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: hydrogen sulfide, malondialdehyde, glutathione

CLC Number:

R318

Fu Zhi-yi, Liu Xing-zhen, Wu Yu-jie, Zhu Tong, Jin Wen-jie . Neuroprotective effects of hydrogen sulfide in rats with acute cauda equina syndrome[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(49): 7914-7918.

Figures/Tables 2

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