Abstract:

BACKGROUND: Resveratrol can inhibit chondrocytes apoptosis in vitro.
OBJECTIVE: To observe the effect of Res on the expression of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha in synoviocytes of osteoarthritis cultured in vitro.
METHODS: After the rabbits were lavaged with clinical equivalent dose of resveratrol, the 10%, 20% and 40% resveratrol-containing serum were prepared, and then co-cultured with the osteoarthritis synoviocyte. The normal rabbit culture serum was as control.
RESULTS AND CONCLUSION: Enzyme linked immunosorbent assay detection and immunocytochemistry results showed that the secretion of interleukin-18, interleukin-1 beta and TNF-α of synoviocyte cultured in vitro in resveratrol-containing serum group was significant lower than that in the non-resveratrol containing serum group (P < 0.01), and with the increasing of resveratrol concentration, the level of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha secretion was gradually reduced (P < 0.01 or P < 0.05). There was a positive correlation between interleukin-18, tumor necrosis factor-alpha and interleukin-1 beta. It indicates that resveratrol can down-regulate the expression of interleukin-18, interleukin-1 beta and tumor necrosis factor-alpha in synoviocyte and reduce the inflammatory reaction.