Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (38): 7031-7036.doi: 10.3969/j.issn.2095-4344.2012.38.001

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Repairing articular cartilage defects in the knee of rabbits using type Ⅰ/Ⅲ-collagen- membrane combined with autologous bone marrow mesenchymal stem cells

He Dong-dong, Zeng Ling-yuan, Xiang Chuan, Wang Yu-ze, Wang Shi-dong, Duan Wang-ping, Lü Jia, Wang Chun-fang, Wei Xiao-chun   

  1. Key Laboratory of Bone & Soft Tissue Injury Repair, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Received:2012-02-10 Revised:2012-03-12 Online:2012-09-16 Published:2012-09-16
  • Contact: Xiang Chuan, Associate professor, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China xcml7275@ yahoo.com.cn
  • About author:He Dong-dong★, Studying for master's degree, Physician, Key Laboratory of Bone & Soft Tissue Injury Repair, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China hedongdong83@ 126.com

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Abstract:

BACKGROUND: Several researches have shown that type Ⅰand type Ⅲ collagens are conducive to the adhesion, proliferation and differentiation of bone marrow mesenchymal stem cells.
OBJECTIVE: To investigate the feasibility of repairing articular cartilage defects in the knee of rabbits using autologous bone marrow mesenchymal stem cells combined with the type Ⅰ/Ⅲ-collagen-membrane.
METHODS: Totally 24 New Zealand rabbits were randomly divided into two groups. A model of full-thickness articular cartilage defects in the bilateral knees with 3.8 mm in diameter and 2 mm in depth was established. The defects filled with the compound of autologous bone marrow mesenchymal stem cells/collagen membrane in 12 rabbits were as the experimental group, while the defects filled with collagen membrane only in another 12 rabbits were as the control group.
RESULTS AND CONCLUSION: The histological staining for the knee femoral specimens showed that the repair tissues mainly were hyaline-like cartilage at week 8, and close to the normal cartilage at week 12 in the experimental group. However, in the control group, the repair tissues mainly were fibrous tissues at week 8, and fiber-based cartilage was repaired at week 12. At 8 and 12 weeks after implantation, the histological scores of the experimental group were significantly higher than those of the control group (P < 0.001). These findings suggest that it is a good way for autologous bone marrow mesenchymal stem cells-Ⅰ/Ⅲ-collagen-membrane to repair articular cartilage defects.

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