Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (11): 1694-1699.doi: 10.3969/j.issn.2095-4344.2014.11.009

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Fluorescent quantitative analysis on the expression of miRNA-34s in human skin keloid tissue

Jin Yu-dan1, Guo Xiao-rui1, Huang Hai-hua2, Lu Ling3, Cai Xiao-jian1, Wang Sui-jiang2   

  1. 1Cosmetic Surgery & Laser Center, Second People’s Hospital of Zhongshan City, Zhongshan 528447, Guangdong Province, China; 2Plastic Surgery Institute, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China; 3Department of Plastic Surgery, First Affiliated Hospital of Gannan Medical College, Ganzhou 341000, Jiangxi Province, China
  • Revised:2014-02-14 Online:2014-03-12 Published:2014-03-12
  • Contact: Jin Yu-dan, Cosmetic Surgery & Laser Center, Second People’s Hospital of Zhongshan City, Zhongshan 528447, Guangdong Province, China
  • About author:Jin Yu-dan, Master, Chief physician, Cosmetic Surgery & Laser Center, Second People’s Hospital of Zhongshan City, Zhongshan 528447, Guangdong Province, China Guo Xiao-rui, Master, Physician, Cosmetic Surgery & Laser Center, Second People’s Hospital of Zhongshan City, Zhongshan 528447, Guangdong Province, China Jin Yu-dan and Guo Xiao-rui contributed equally to this work.
  • Supported by:

    grants from Science and Technology Plan of Zhongshan City, No. 20122A041

Abstract:

BACKGROUND: Understanding the difference of miRNA-34s expression in normal tissue and tumor tissue will contribute to screen out a miRNA with high sensitivity as the specific tumor molecular marker.

OBJECTIVE: To investigate the differential expression of miRNA-34s (miR-34a/b/c) between normal skin and keloid tissue using real-time fluorescent quantitative PCR, and to evaluate the role and mechanisms of miRNA-34s in keloid formation and development.
METHODS: Ten cases of keloid tissue and two cases of normal skin tissue were collected as specimens. Total RNAs were extracted from keloid and nomal skin tissue by Trizol method, and miRNA-34s were further isolated by Ambion’s miRNA Isolation Kit. Real-time fluorescent quantitative PCR was applied to verify expression levels of microRNA-34s (miR-34a/b/c) in keloid tissue and normal skin tissue.
RESULTS AND CONCLUSION: miRNA-34s (miRNA-34a/b/c) expression was down-regulated in keloid tissue compared with normal skin tissue (P < 0.01). The findings showed that miRNA-34s (miRNA-34a/b/c) are involved in keloid formation and development, and down-regulation of the family member may result in neoplastic growth of keloid.


中国组织工程研究
杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程


全文链接:

Key words: microRNAs, keloid, reverse transcriptase polymerase chain reaction

CLC Number: