Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1397-1401.doi: 10.3969/j.issn.1673-8225.2011.08.016

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Corrosion resistance and biocompatibility of binary magnesium alloys in cell culture medium

Yang Chun-xi1,2, Zheng Yu-feng3, Gu Xue-nan3, Yuan Guang-yin4, Zhang Jia4, Dai Ke-rong2   

  1. 1Department of Orthopedics, Shanghai Tenth People’s Hospital (Tenth People’s Hospital of Tongji University), Shanghai  200072, China
    2Department of Orthopedics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
    3Department of Advanced Materials and Nanotechnology, College of Engineering, Peking University, Beijing  100871, China
    4National Engineering Research Center of Light Alloys Net Forming, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai   200240, China
  • Received:2010-08-08 Revised:2010-11-07 Online:2011-02-19 Published:2011-02-19
  • Contact: Dai Ke-rong, Chief physician, Professor, Department of Orthopedics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China krdai@163.com
  • About author:Yang Chun-xi☆, Doctor, Attending physician, Department of Orthopedics, Shanghai Tenth People’s Hospital (Tenth People’s Hospital of Tongji University), Shanghai 200072, China; Department of Orthopedics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China chunxi_yang@163.com
  • Supported by:

    Shanghai Key Laboratory of Orthopedic Implants, No. 08DZ2230330*; Key Disciplines of Shanghai Municipal Education Commission, No. J50206*; the National Basic Research Program of China (973 Program), No. 2007CB936101*; Shanghai Rising-Star Program, No. 08QA14045*; the National Natural Science Foundation of China, No. 30600628*

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Abstract:

BACKGROUND: Degrading magnesium and magnesium alloys are a new class of implant materials suitable for orthopedic surgery due to its bone conductibility and degradability, so further research on the evaluation of magnesium is very necessary.
OBJECTIVE: To determine the degradability of magnesium and binary magnesium alloys in cell culture medium and their influence on the viability of adult human bone marrow mesenchymal stem cells.
METHODS: Eight alloying elements Al, Ca, Mn, Si, Sn, Y, Zn and Zr were added into magnesium individually to fabricate binary Mg-1X (wt.%) alloys. Pure magnesium and eight binary magnesium alloys were immersed in α-MEM (containing 10% fetal bovine serum) to prepare extracts. The pH values of the extraction media were measured. Magnesium and alloy element ions in the extraction medium were analyzed using inductively coupled plasma-atomic emission spectrometry. Adult human bone marrow mesenchymal stem cells were cultured in extracts of 100%, 50%, 25% magnesium and eight binary magnesium alloys for 1, 3, 5, 7 days. The indirect effects of magnesium alloys on viability of adult human bone marrow mesenchymal stem cells were detected using Alamar Blue? cell viability reagent.
RESULTS AND CONCLUSION: Mg-Ca and Mg-Y alloys showed poor corrosion resistance. The Mg2+ concentration in liquid was (408.0±37.9) mg/L and (351±15.3) mg/L, respectively, while pH value was 8.87±0.19 and 8.84±0.15. Next is Mg-Zr alloy; other binary alloys were equal to pure Mg regarding corrosion resistance. The magnesium and eight binary magnesium alloys extracts at 100% concentration significantly inhibited the viability of adult human bone marrow mesenchymal stem cells. But the extracts did not inhibit the cell viability while Mg2+ concentration was ≤ 110 mg/L and pH value of 7.35-7.65.

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