Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (8): 1392-1396.doi: 10.3969/j.issn.1673-8225.2011.08.015

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Preparation of bone morphogenetic protein-7 peptide/chitosan/nanometer hydroxyapatite/collagen biomimetic composite and its cytocompatibility

Feng Bo1, Hu Dong-xu2, Zhang Yang-de2, Wu Yu-chi1   

  1. 1Institute of Biomedical Engineering, Central South University, Orthopaedics Department of the Third Affiliated Hospital of Inner Mongolia Medical College, Baotou  014010, Inner Mongolia Autonomous Region, China
    2National Hepatobiliary & Enteric Surgery Research Center,  Ministry of Health, Changsha  410008, Hunan Province, China
  • Received:2010-11-20 Revised:2010-12-25 Online:2011-02-19 Published:2011-02-19
  • Contact: Zhang Yang-de, Doctoral supervisor, Chief physician, Professor, National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Changsha 410008, Hunan Province, China zyd@2118.cn
  • About author:Feng Bo☆, Studying for doctorate, Chief physician, Institute of Biomedical Engineering, Central South University, Orthopaedics Department of the Third Affiliated Hospital of Inner Mongolia Medical College, Baotou 014010, Inner Mongolia Autonomous Region, China bb0472111@yahoo.com.cn

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Abstract:

BACKGROUND: The commonly used scaffold materials in bone tissue engineering are inorganic materials, organic polymer materials and natural derived materials, these materials have their advantages and disadvantages. In order to give full play to the advantages of various materials and make up for its shortcomings, composite materials are currently applied for the preparation of composite scaffold.
OBJECTIVE: To prepare bone morphogenetic protein-7 (BMP-7) peptide/chitosan/nanometer hydroxyapatite/collagen biomimetic scaffold, and to explore the effect of it on the adhesion, proliferation and differentiation of bone marrow stromal cells (BMSCs).
METHODS: The chitosan/nanometer hydroxyapatite/collagen composite scaffold was prepared, and the microcosmic appearance of the composite was observed by scanning electron microscope. The BMP-7 peptide was introduced into the composite by vacuum adsorption. The in vitro release of BMP-7 peptide from the scaffold was detected by using high performance liquid chromatography. The BMSCs were seeded onto the composite scaffold loaded BMP-7 peptide and the unloaded composites served as controls. The BMSCs proliferation, adhesion, growth morphology and alkaline phosphatase activities on the scaffold surface were analyzed.
RESULTS AND CONCLUSION: The scaffold of chitosan/nanometer hydroxyapatite/collagen composite was porous at the pore size of 10-100 µm. The releasing character of BMP-7 peptide belonged to slow release. There was no significant difference on the proliferation of the BMSCs between the experimental group and the control group (P > 0.05), but the abilities of adhesion and osteogenic differentiation of the BMSCs in the experimental group were significantly greater than those in the control group (P < 0.05). BMP-7 peptide/chitosan/nanometer hydroxyapatite/collagen biomimetic composite scaffold is a kind of ideal scaffold materials for bone tissue engineering and has good biocompatibility.

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