Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (42): 7843-7848.doi: 10.3969/j.issn.1673-8225.2010.42.013

Previous Articles     Next Articles

Cellular compatibility of strontium-doped calcium polyphosphate

Song Yan-cheng1, Zeng Chun1, Jin Wen-tao1, Cai Dao-zhang1, Wan Chang-xiu2, Pei Fu-xing3　   

  1. 1 Department of Orthopaedics, Third Hospital Affiliated to Sun Yat-sen University, Guangzhou   510630, Guangdong Province, China; 2 College of Polymer Science and Engineering, Sichuan University, Chengdu   610041, Sichuan Province, China; 3 Department of Orthopaedics, West China Hospital of Sichuan University, Chengdu   610041, Sichuan Province, China
  • Online:2010-10-15 Published:2010-10-15
  • Contact: Cai Dao-zhang, Professor, Department of Orthopaedics, Third Hospital Affiliated to Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
  • About author:Song Yan-cheng☆, Doctor, Attending physician, Department of Orthopaedics, Third Hospital Affiliated to Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China songyancheng@21cn.com
  • Supported by:

     the National Natural Science Foundation of China, No. 50472091*

PDF

365

Abstract:

BACKGROUND: It is reported that strontium-doped calcium polyphosphate with controllable degradability and enough mechanical strength could be synthesized by adding trace strontium into biodegradable calcium polyphosphate due to the modulation of material structure, such as pore size and porosity.
OBJECTIVE: To study the cellular compatibility of strontium-doped calcium polyphosphate to osteoblasts.
METHODS: The rabbit osteoblasts were co-cultured in vitro on the strontium-doped calcium polyphosphate, their morphology and biology were studied. MTT assay was applied to determine the osteoblast vitality in strontium-doped calcium polyphosphate and alkaline phosphatase activities were detected.
RESULTS AND CONCLUSION: Under inverted microscope, scanning electron microscopy and confocal laser microscopy, the co-cultured osteoblast adhered, proliferated and grew normally on the surface and pores of strontium-doped calcium polyphosphate, and secreted extracellular matrix and massive microvillus on the cell surface. The osteoblast grew well on strontium-doped calcium polyphosphate; meanwhile, cellular function was shown to be well by MTT assay and alkaline phosphatase activities, 1% strontium-doped calcium polyphosphate achieve the maximum of cellular proliferation activity and alkaline phosphatase activities, no toxicity on osteoblasts and good cellular compatibility.

CLC Number: