Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (28): 5160-5164.doi: 10.3969/j.issn.2095-4344.2012.28.007

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Effects of prostaglandin E2 on the migration of posterior cruciate ligament fibroblasts and synovial cells

Chen Rong-fu1, Wang Chun-li2, Xie Jing2, Huang Wei1, Liang Xi1, KL Paul Sung2, 3○, Fu Chun-feng1, Chen Cheng1   

  1. 1Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;
    2College of Bioengineering, Chongqing University, Chongqing 400030, China;
    3Department of Orthopedics and Bioengineering, University of California, Califonia CA 92093-0412, USA
  • Received:2012-03-07 Revised:2012-05-07 Online:2012-07-08 Published:2012-07-08
  • Contact: Huang Wei, Doctor, Professor, Chief physician, Master’s supervisor, Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China huangwei68huangwei @163.com
  • About author:Chen Rong-fu★, Master, Physician, Department of Orthopedics, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China 271500790@qq.com

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Abstract:

BACKGROUND: Cells migration is one of the important steps in the process of wound repair. But the effects of prostaglandin E2 on the migration of posterior cruciate ligament fibroblasts and synovial cells remain unclear.
OBJECTIVE: To observe the effects of prostaglandin E2 on the migration of posterior cruciate ligament fibroblasts and synovial cells under mono-culture and Transwell co-culture in vitro.
METHODS: Posterior cruciate ligament fibroblasts and synovial cells were mono-cultured and co-cultured by Transwell. Then the mono-cultured cells or co-cultured cells were treated by 10 μg/L prostaglandin E2. Cell growth status was observed and compared. Cell migration was assessed by wound healing assay at 24 hours after treatment.
RESULTS AND CONCLUSION: The migration rates of posterior cruciate ligament fibroblasts and synovial cells under co-culture were increased by 128% and 48% compared with the mono-cultured cells. Prostaglandin E2 decreased the migration rates of the mono-cultured posterior cruciate ligament fibroblasts and synovial cells by 28% and 14% compared with the mono-culture control, and also decreased the migration rates of the co-cultured cells by 37% and 24% compared with the co-culture group. These results indicate that prostaglandin E2 inhibited the migration of both posterior cruciate ligament fibroblasts and synovial cells, especially stronger in co-cultured posterior cruciate ligament fibroblasts.

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