Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (46): 8608-.doi: 10.3969/j.issn.1673-8225.2010.46.014

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Construction of rat models with frostbite to varying degrees

Wang Peng, Li Jian   

  1. Jiangxi Provincial Key Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-11-12 Published:2010-11-12
  • Contact: Li Jian, Doctor, Investigator, Jiangxi Provincial Key Laboratory of Molecular Medicine, Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China jeniferjian@gmail.com
  • Supported by:

    the National Science & Technology Pillar Program during the Eleventh Five-Year Plan Period, No. 2008BAI68B06*; the National Natural Science Foundation of China, No. 30760261*

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Abstract:

BACKGROUND: There is not a specific treating method for frostbite. A frostbite model has great significance in researching the mechanism of frostbite and its repair. OBJECTIVE: To construct rat models with varying degrees frostbite, and to explore a new method for frostbite model preparation.  
METHODS: Coins were immersed into liquid nitrogen to reach -196℃ and then nestled up to the skin of rat for 3, 5 and 10 seconds. Specimens of frostbite tissue were harvested after 24 hours and performed routine hematoxylin-eosin staining. Changes of histopathologic structure after frostbite were also observed under a microscope.
RESULTS AND CONCLUSION: For light frostbite (3 seconds), only superficial epidermis was damaged, underlayer appeared normal. For midrange frostbite (5 seconds), full-thickness of epidermis, including the underlayer, was damaged, however, deeplayer of dermis and hair follicle were intact. For severe frostbite (10 seconds), deeplayer of dermis and hair follicle were damaged wholly. Light frostbite and midrange frostbite could be used as a standard to construct animal models.

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